Bone metastases: prognostic applications of cytometric DNA analysis.

Objective: To examine DNA parameters as prognostic factors for developing metastases.

Study Design: Image cytometry was used to determine DNA content of 21 tumors and 28 metastases. DNA ploidy status, 2c deviation index (2cDI) and DNA malignancy grade (DNA-MG) (based on the variation of nuclear DNA content of tumor cells around the normal DNA [2c] peak) were examined for their prognostic value.