Alveolar bone healing patterns in chronic kidney failure and kidney transplant recipients: A pixel intensity and fractal analyses.

Aims: To assess and compare radiographically the alveolar bone after tooth extractions in individuals with chronic kidney failure undergoing hemodialysis (CKFh), those submitted to kidney transplantation (KT), and those without kidney disease (CG) by using fractal analysis (FA) and pixel intensity (PI).

Methods And Results: Periapical radiographs of 48 CKFh individuals (87 extracted teeth), 12 KT individuals (26 extracted teeth and 29 control individuals [76 extracted teeth] were analyzed at 7 and 60 days after tooth extraction. Fractal dimension (FD) and PI were assessed to evaluate the alveolar trabecular bone structural complexity and mineral content.

[Frequency and reasons for missed nursing care: a cross-sectional study in Veneto Region hospital].

Unlabelled: . Frequency and reasons for missed nursing care: a cross-sectional study in Veneto Region hospitals.

Introduction: Missed nursing care (MNC) encompasses clinical, relational, and emotional aspects of care that are neglected, delayed, or incomplete as performed by nurses.

Long-Term Effect of Crop Succession Systems on Soil Chemical and Physical Attributes and Soybean Yield.

Most soybean producers in the Cerrado biome use the direct seeding system, making it essential to cultivate cash or cover crops in the off-season, to promote soil protection, as well as increase organic matter, which is directly related to improvements in the chemical and physical characteristics of these soils. In this sense, this work was conducted in Jataí, state of Goias, Brazil, to evaluate the physical-chemical attributes of the soil and the performance of soybeans cultivated in different crop succession systems cultivated for 6 years in the region of Jataí, GO. The experimental design was randomized blocks with four plots and four replications; the crops that followed soybeans were arranged as follows: T1-corn (); T2-pearl millet (); T3-; and T4-corn + .

Mechanical Properties and Failure Mode of Proximal Screw Fixation Technique Using Locking Compression Plate for Proximal Interphalangeal Arthrodesis in Horses: An Ex Vivo Study.

Objective:  The aim of this study was to measure the mechanical properties and failure mode of an model of proximal interphalangeal arthrodesis in horses with different methods of proximal screw fixation using a locking compression plate.

Study Design:  Pastern joint arthrodesis with a 3-hole 4.5-mm narrow locking compression plate was performed on 20 forelimbs of equine cadavers, randomized into four groups based on the fixation method and type of proximal screw used: unicortical cortex, bicortical cortex, unicortical locking, bicortical locking.

Gingival proteomics reveals the role of TGF beta and YAP/TAZ signaling in Raine syndrome fibrosis.

Raine syndrome (RNS) is a rare autosomal recessive osteosclerotic dysplasia. RNS is caused by loss-of-function disease-causative variants of the FAM20C gene that encodes a kinase that phosphorylates most of the secreted proteins found in the body fluids and extracellular matrix. The most common RNS clinical features are generalized osteosclerosis, facial dysmorphism, intracerebral calcifications and respiratory defects.

Accuracy of linear measurements for implant planning based on low-dose cone beam CT protocols: a systematic review and meta-analysis.

Objectives: The aim of this systematic review was to verify the accuracy of linear measurements performed on low-dose CBCT protocols for implant planning, in comparison with those performed on standard and high-resolution CBCT protocols.

Methods: The literature search included four databases (Pubmed, Web of Science, Embase, and Scopus). Two reviewers independently screened titles/abstracts and full texts according to eligibility criteria, extracted the data, and examined the methodological quality.

[The education of specialized nurses' aides: current scenario and future perspectives].

Unlabelled: . The education of specialized nurses' aides: current scenario and future perspectives.

Introduction: The complementary training in health care of the nurse aides (NAs), launched in Italy in 2003, has little impact on health care organisations.

The solute carrier SLC25A17 sustains peroxisomal redox homeostasis in diverse mammalian cell lines.

Despite the crucial role of peroxisomes in cellular redox maintenance, little is known about how these organelles transport redox metabolites across their membrane. In this study, we sought to assess potential associations between the cellular redox landscape and the human peroxisomal solute carrier SLC25A17, also known as PMP34. This carrier has been reported to function as a counter-exchanger of adenine-containing cofactors such as coenzyme A (CoA), dephospho-CoA, flavin adenine dinucleotide, nicotinamide adenine dinucleotide (NAD), adenosine 3',5'-diphosphate, flavin mononucleotide, and adenosine monophosphate.

Functional Analysis of GSTK1 in Peroxisomal Redox Homeostasis in HEK-293 Cells.

Peroxisomes serve as important centers for cellular redox metabolism and communication. However, fundamental gaps remain in our understanding of how the peroxisomal redox equilibrium is maintained. In particular, very little is known about the function of the nonenzymatic antioxidant glutathione in the peroxisome interior and how the glutathione antioxidant system balances with peroxisomal protein thiols.

Human peroxisomal NAD/NADH homeostasis is regulated by two independent NAD(H) shuttle systems.

Reduced (NADH) and oxidized (NAD) nicotinamide adenine dinucleotides are ubiquitous hydride-donating/accepting cofactors that are essential for cellular bioenergetics. Peroxisomes are single-membrane-bounded organelles that are involved in multiple lipid metabolism pathways, including beta-oxidation of fatty acids, and which contain several NAD(H)-dependent enzymes. Although maintenance of NAD(H) homeostasis in peroxisomes is considered essential for peroxisomal beta-oxidation, little is known about the regulation thereof.

[The resignations among nurses in the Veneto healthcare institutions. A retrospective study].

Unlabelled: . The resignations among nurses in the Veneto healthcare institutions. A retrospective study.

Enhanced Levels of Peroxisome-Derived HO Do Not Induce Pexophagy but Impair Autophagic Flux in HEK-293 and HeLa Cells.

Peroxisomes are functionally specialized organelles that harbor multiple hydrogen peroxide (HO)-producing and -degrading enzymes. Given that this oxidant functions as a major redox signaling agent, peroxisomes have the intrinsic ability to mediate and modulate HO-driven processes, including autophagy. However, it remains unclear whether changes in peroxisomal HO (po-HO) emission impact the autophagic process and to which extent peroxisomes with a disturbed HO metabolism are selectively eliminated through a process called "pexophagy".

Assessment of the Peroxisomal Redox State in Living Cells Using NADPH- and NAD/NADH-Specific Fluorescent Protein Sensors.

The pyridine nucleotides NAD(H) and NADP(H) are key molecules in cellular metabolism, and measuring their levels and oxidation states with spatiotemporal precision is of great value in biomedical research. Traditional methods to assess the redox state of these metabolites are intrusive and prohibit live-cell quantifications. This obstacle was surpassed by the development of genetically encoded fluorescent biosensors enabling dynamic measurements with subcellular resolution in living cells.

Identification of Peroxisome-Derived Hydrogen Peroxide-Sensitive Target Proteins Using a YAP1C-Based Genetic Probe.

As the reversible oxidation of protein cysteine thiols is an important mechanism in signal transduction, it is essential to have access to experimental approaches that allow for spatiotemporal indexing of the cellular sulfenome in response to local changes in HO levels. Here, we provide a step-by-step guide for enriching and identifying the sulfenome of mammalian cells at the subcellular level in response to peroxisome-derived HO by the combined use of (i) a previously developed cell line in which peroxisomal HO production can be induced in a time- and dose-dependent manner; (ii) YAP1C, a genetically encoded yeast AP-1-like transcription factor-based probe that specifically reacts with S-sulfenylated cysteines and traps them through mixed disulfide bonds; and (iii) mass spectrometry. Given that this approach includes differential labeling of reduced and reversibly oxidized cysteine residues, it can also provide additional information on the positions of the modified cysteines.

Quantitative and qualitative comparisons of pulp cavity volumes produced by cone beam computed tomography and micro-computed tomography through semiautomatic segmentation: An ex vivo investigation.

Objective: The aim of this study was to measure the volume and visually assess 3-dimensional (3D) virtual models of pulp cavities obtained through semiautomatic segmentation on images from 6 cone beam computed tomography (CBCT) units compared with the reference standard of micro-CT.

Study Design: Fifteen mandibular premolar teeth were scanned with 6 CBCT units: Prexion 3D Elite, i-CAT Next Generation, NewTom 5G, Cranex 3D, 3Shape X1, and Orthophos SL 3D, using the smallest available field of view and highest resolution settings. Pulp cavity volumes were quantitatively assessed by 2 calibrated examiners.

Peroxisome-Derived Hydrogen Peroxide Modulates the Sulfenylation Profiles of Key Redox Signaling Proteins in Flp-In T-REx 293 Cells.

The involvement of peroxisomes in cellular hydrogen peroxide (HO) metabolism has been a central theme since their first biochemical characterization by Christian de Duve in 1965. While the role of HO substantially changed from an exclusively toxic molecule to a signaling messenger, the regulatory role of peroxisomes in these signaling events is still largely underappreciated. This is mainly because the number of known protein targets of peroxisome-derived HO is rather limited and testing of specific targets is predominantly based on knowledge previously gathered in related fields of research.

Colloidal stability of milk: reinterpretation of alcohol test results by digital microscopy.

In this research communication we propose a new approach by portable digital microscopy with a 200× objective to improve the visualization of microparticles of pasteurized milk submitted to the alcohol test. Not only did the method reduce the subjectivity of the readings, but also generated high resolution images of the microparticles, which allows the creation of a specific image pattern for each type of final product. In comparison to a control pasteurized milk treatment, the results confirmed the effect and the specificity of added salts (sodium citrate, disodium phosphate or their combination) on the stability of the milk to the alcohol test.

Mitochondrial and metabolic remodelling in human skin fibroblasts in response to glucose availability.

Cell culture conditions highly influence cell metabolism in vitro. This is relevant for preclinical assays, for which fibroblasts are an interesting cell model, with applications in regenerative medicine, diagnostics and therapeutic development for personalized medicine, and the validation of ingredients for cosmetics. Given these cells' short lifespan in culture, we aimed to identify the best cell culture conditions and promising markers to study mitochondrial health and stress in normal human dermal fibroblasts (NHDF).

The Peroxisome-Autophagy Redox Connection: A Double-Edged Sword?

Peroxisomes harbor numerous enzymes that can produce or degrade hydrogen peroxide (HO). Depending on its local concentration and environment, this oxidant can function as a redox signaling molecule or cause stochastic oxidative damage. Currently, it is well-accepted that dysfunctional peroxisomes are selectively removed by the autophagy-lysosome pathway.

Efficacy of an iodoform-based filling material for pulpectomy of primary teeth: A 24-month non-inferiority randomized clinical trial.

Aim: The aim of this non-inferiority randomized clinical trial was to compare the efficacy of an iodoform-based paste (Guedes-Pinto -(GP)) as a filling material in pulpectomies of primary teeth, and a standard material composed by calcium hydroxide and iodoform (CaOH/Iodof paste; Vitapex ).

Design: A total of 104 teeth from 61 children (3-8 years old) were randomly allocated to two groups according to filling materials. Children were followed up for 24 months.

[Conclusions and reflections for planning not based on average numbers].

. Conclusions and reflections for planning not based on average numbers. Planning for the number of Family and community nurses (FCNs) needed cannot be based on average estimates issued at the national level.

[Family nurses in Italy: an explorative survey].

Unlabelled: . Family nurses in Italy: an explorative survey.

Introduction: The Family and Community Nurses (FCN) are unevenly distributed throughout Italy.

[Family and community nursing: overview of practice in Europe].

. Family and Community nursing: overview of practice in Europe. In this contribution, an overview of the role and functions of the family nurse in some European countries is presented.

Radiographic analysis of the management of tooth extractions in head and neck-irradiated patients: a case series.

Tooth extraction after head and neck radiotherapy exposes patients to an increased risk for osteoradionecrosis of the jaw. This study reports the results of a radiographic analysis of bone neoformation after tooth extraction in a case series of patients who underwent radiation therapy. No patients developed osteoradionecrosis within a follow-up of 1 year.