Publications by authors named "Claudia Trigo Pedroso Moraes"
Genetic variability in G2 and F2 region between biological clones of human respiratory syncytial virus with or without host immune selection pressure.
Mem Inst Oswaldo Cruz
February 2015
Article Synopsis
- Human respiratory syncytial virus (HRSV) is a significant respiratory illness affecting young children, making vaccine development challenging due to host immunity and the virus's genetic variability.
- This study identified differences in HRSV biological clones from clinical samples, noting specific mutations in the F and G genes in relation to serum from convalescent children and their mothers.
- Findings suggest that host antibodies may influence the selection of viral sequences, complicating the creation of effective vaccines by contributing to the virus's evolutionary process.
Molecular epidemiology of the SH (small hydrophobic) gene of human respiratory syncytial virus (HRSV), over 2 consecutive years.
Virus Res
January 2012
Human respiratory syncytial virus (HRSV) strains were isolated from nasopharyngeal aspirates collected from 965 children between 2004 and 2005, yielding 424 positive samples. We sequenced the small hydrophobic protein (SH) gene of 117 strains and compared them with other viruses identified worldwide. Phylogenetic analysis showed a low genetic variability among the isolates but allowed us to classify the viruses into different genotypes for both groups, HRSVA and HRSVB.
View Article and Find Full Text PDFHuman respiratory syncytial virus (HRSV) is the main cause of acute lower respiratory tract infections in infants and children. Rapid diagnosis is required to permit appropriate care and treatment and to avoid unnecessary antibiotic use. Reverse transcriptase (RT-PCR) and indirect immunofluorescence assay (IFA) methods have been considered important tools for virus detection due to their high sensitivity and specificity.
View Article and Find Full Text PDFArticle Synopsis
- The study investigated the effectiveness of three cell lines (HEp-2, NCI-H292, HeLa-I) in isolating Human Respiratory Syncytial Virus (HRSV) from patients with respiratory issues.
- HRSV isolation rates were 46% in HeLa-I, 48% in HEp-2, and 36.3% in NCI-H292, with immunofluorescence being the gold standard, achieving a 53% positive rate.
- Combining all three cell lines improved sensitivity for detecting HRSV to 73.2%, with HeLa-I showing similar effectiveness to HEp-2, suggesting that using multiple cell