Calcium-binding proteins that are type B″ regulatory subunits of phosphatase 2A in Giardia intestinalis.

Giardia intestinalis is a protozoan parasite that colonizes the upper part of the small intestine of its mammalian hosts. The trophozoite, which is the replicative stage, has a complex cytoskeleton that allows it to move and adhere to intestinal cells. It has been proposed that protein phosphatase 2A (PP2A) participates in the regulation of changes to the parasite cytoskeleton during its life cycle.

In silico analysis of the EF-hand proteins in the genome of Giardia intestinalis assembly A.

Giardia intestinalis is a parasite that inhabits the small intestine of humans and other mammals, causing a disease that can manifest itself with acute diarrhea. This parasite is an early divergent eukaryote with a compact genome and a life cycle composed of two distinct cell types: the trophozoite, the replicative form, and the cyst, the infectious form. Signal transduction pathways implicated in differentiation processes of G.

Experimental and bioinformatic characterization of CaBP2933 an EF-Hand protein of Giardia intestinalis.

Giardia intestinalis is a parasite that inhabits the small intestine of humans. This parasite is a divergent eukaryote with a compact genome. The calcium ion is an essential messenger in cell signaling.

[Identification of the gene sequence of telomerase catalytic subunit in Plasmodium falciparum].

Introduction: The enzyme telomerase regulates telomere length by synthesis of telomeric repeats to compensate for telomeric loss in each DNA replication cycle. Therefore, telomerase is a potential target to block growth of cells with high replication rates. In Plasmodium falciparum, telomerase activity has been documented, but little information on its structure and role.

Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method.

A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10 to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.

Genetic diversity of Plasmodium falciparum field samples from an isolated Colombian village.

Colombian field isolates of Plasmodium falciparum were analyzed for genetic diversity. Fifty-three samples were collected as thick smears from patients living in Panguí, an isolated area with low migration. While the samples were being collected, Panguí was experiencing an epidemic outbreak of malaria.