Differential expression of peptidases in Strigomonas culicis wild-type and aposymbiotic strains: from proteomic data to proteolytic activity.

Background: Strigomonas culicis is a monoxenic trypanosomatid parasite of insects that naturally contains an endosymbiotic bacterium. The aposymbiotic strain can be obtained, making this strain a model for evolutive research about organelle origins. In addition, S.

Susceptibility of Axenic Amastigotes to the Calpain Inhibitor MDL28170.

Leishmaniasis encompasses a group of neglected diseases caused by flagellated protozoa belonging to the genus, associated with high morbidity and mortality. The search for compounds with anti- activity that exhibit lower toxicity and can overcome the emergence of resistant strains remains a significant goal. In this context, the calpain inhibitor MDL28170 has previously demonstrated deleterious effects against promastigote forms of , which led us to investigate its role on axenic amastigote forms.

Heme metabolism in Strigomonas culicis: Implications of HO resistance induction and symbiont elimination.

Monoxenous trypanosomatid Strigomonas culicis harbors an endosymbiotic bacterium, which enables the protozoa to survive without heme supplementation. The impact of HO resistance and symbiont elimination on intracellular heme and Fe availability was analyzed through a comparison of WT strain with both WT HO-resistant (WTR) and aposymbiotic (Apo) protozoa. The relative quantification of the heme biosynthetic pathway through label-free parallel reaction monitoring targeted mass spectrometry revealed that HO resistance does not influence the abundance of tryptic peptides.

hsp70 PCR-RFLP as an alternative tool to identify Sauroleishmania species.

Sauroleishmania spp. comprises one of the four Leishmania subgenera, which has been historically considered a non-pathogenic protozoan of reptiles. However, some strains appear to be transiently infective to mammals, and recent findings have detected these parasites in dogs and humans in areas where leishmaniasis is endemic.