Production of a recombinant membrane protein in an strain for the whole cell biosynthesis of phenylacetic acids.

The styrene oxide isomerase (SOI) represents a membrane-bound enzyme of the microbial styrene degradation pathway and has been discussed as promising biocatalyst. It catalyzes the isomerization of styrene oxide to phenylacetaldehyde. In this study a gene, which encodes the SOI of 1CP, was optimized for optimal expression in BL21(DE3) pLysS.