Lysoquinone-TH1, a New Polyphenolic Tridecaketide Produced by Expressing the Lysolipin Minimal PKS II in .

The structural repertoire of bioactive naphthacene quinones is expanded by engineering to express the lysolipin minimal polyketide synthase II (PKS II) genes from Tü 4042 () with the corresponding cyclase genes . Fermentation of the recombinant strain revealed the two new polyaromatic tridecaketides lysoquinone-TH1 (, identified) and TH2 (, postulated structure) as engineered congeners of the dodecaketide lysolipin (). The chemical structure of , a benzo[a]naphthacene-8,13-dione, was elucidated by NMR and HR-MS and confirmed by feeding experiments with [1,2-C₂]-labeled acetate.