The electrical behaviour of rat connexin46 gap junction channels expressed in transfected HeLa cells.

Pairs of human HeLa cells expressing rat connexin46 were used to study the electrical properties of gap junction channels with the dual voltage-clamp method. The steady-state conductance ( g(j,ss)) had a bell-shaped dependence on transjunctional voltage ( V(j)). The parameters of the Boltzmann fit were: V(j,0)=42 mV, g(j,min)=0.