Publications by authors named "Claudia Cano-Ramirez"
The acoustic communication system (ACS) in bark beetles has been studied mainly in species of the genera , and . Specifically, ACS of the roundheaded pine beetle, , has been little studied. In this study, we described the stridulatory apparatus of this beetle using optical and scanning electron microscopy and recorded the call types produced by males in three behavioral contexts: stress, female-male-, and male-male interactions.
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- * The study focused on the evolutionary functions of CYP6DE and CYP6DJ subfamilies, utilizing phylogenetic analysis and molecular docking to examine how these enzymes interact with various monoterpenes.
- * Findings revealed multiple phylogenetic subgroups with distinct evolutionary patterns; CYP6DE exhibits type I and II divergence, enhancing detoxification abilities, while CYP6DJ operates under strong constraints, highlighting the specialization and adaptability of these enzymes in bark beetles.
Article Synopsis
- - Species from a specific genus are key components of the gut microbiome of bark beetles, significant pests affecting pine forests in North America and Eurasia.
- - Researchers examined 300 bacterial isolates from the beetle gut, ultimately focusing on 10 which underwent detailed analysis including genome sequencing and phylogenetic studies to confirm their classification.
- - Findings indicated that two bacterial strains, ChDrAdgB13 and JaDmexAd06, possess unique features like peritrichous flagella and fimbriae, confirming they form a dominant ecotype within the beetles' gut, crucial throughout all their life stages.
Improvement of Laccase Production by Co3Bag1. Enhancing the Bio-Catalytic Performance of the Native Thermophilic LacA via Immobilization in Copper Alginate Gel Beads.
Marina Gutiérrez-Antón Alejandro Santiago-Hernández Johan Rodríguez-Mendoza Claudia Cano-Ramírez Ismael Bustos-Jaimes
J Fungi (Basel)
February 2023
A 32-fold increase in laccase activity production by the thermophilic biomass-degrading fungus Co3Bag1 was achieved when the microorganism was grown on a modified medium containing fructose, sodium nitrate, and copper. A 70 kDa laccase (LacA), produced under the above conditions, was purified, immobilized in copper alginate gel beads, and characterized. LacA, both free and immobilized enzymes, exhibited optimal activity at pH 3.
View Article and Find Full Text PDFTtCel7A: A Native Thermophilic Bifunctional Cellulose/Xylanase Exogluclanase from the Thermophilic Biomass-Degrading Fungus Co3Bag1, and Its Application in Enzymatic Hydrolysis of Agroindustrial Derivatives.
Azucena López-López Alejandro Santiago-Hernández Maribel Cayetano-Cruz Yolanda García-Huante Jorge E Campos
J Fungi (Basel)
January 2023
Article Synopsis
- The thermophilic fungus Co3Bag1 produces TtCel7A, a bifunctional enzyme that acts as both a cellulase and xylanase, crucial for breaking down biomass.
- TtCel7A functions optimally at a pH of 5.5 and temperatures of 60°C for cellulase activity and 50°C for xylanase activity, with varying stability at higher temperatures.
- The enzyme effectively hydrolyzes various substrates, producing glucose and cellobiose, making it a promising candidate for industrial applications in biomass degradation.
Article Synopsis
- Sp. ChDrAdgB13, a gut bacteria associated with destructive bark beetles, was studied for its enzyme capabilities related to carbohydrate metabolism, specifically focusing on xylan hydrolysis.
- The study identified and characterized a bifunctional enzyme called R13 Fae, which involves glycosyl hydrolases that show activity on xylan and ferulic acid, demonstrating its nutritional potential for the beetle and gut microbes.
- Biochemical analysis revealed that R13 Fae operates best at pH 6.0 and 25°C, has a long half-life of 23 days, and is stable in the presence of most metal ions, indicating its importance for the beetle's survival and interaction with gut microbi
The Bark Beetle (Curculionidae: Scolytinae) Has Digestive Capacity to Degrade Complex Substrates: Functional Characterization and Heterologous Expression of an α-Amylase.
L Viridiana Soto-Robles María Fernanda López Verónica Torres-Banda Claudia Cano-Ramírez Gabriel Obregón-Molina
Int J Mol Sci
December 2020
Article Synopsis
- - Bark beetles play a crucial role in coniferous forests by helping with regeneration, succession, and material recycling by colonizing and killing stressed or damaged pine trees.
- - The study focused on understanding a specific α-amylase enzyme (AmyDr) found in these beetles, which helps them digest starch; it identified a gene coding for a protein that has a unique mutation affecting its binding sites.
- - Results showed that the enzyme displayed higher activity in the gut compared to other body parts, suggesting that starch digestion is primarily done in the midgut, with its activity influenced by the beetle's developmental stages and starch availability in their host trees.
The Differential Expression of Mevalonate Pathway Genes in the Gut of the Bark Beetle (Curculionidae: Scolytinae) Is Unrelated to the de Novo Synthesis of Terpenoid Pheromones.
Laura Elisa Sarabia María Fernanda López Gabriel Obregón-Molina Claudia Cano-Ramírez Guillermo Sánchez-Martínez
Int J Mol Sci
August 2019
Bark beetles commonly produce de novo terpenoid pheromones using precursors synthesized through the mevalonate pathway. This process is regulated by Juvenile Hormone III (JH III). In this work, the expression levels of mevalonate pathway genes were quantified after phloem feeding-to induce the endogenous synthesis of JH III-and after the topical application of a JH III solution.
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- Bark beetles are major pests of conifer trees, with 20 species found in North and Central America and Eurasia, yet little is known about their gut bacteria across different locations.
- This study uses advanced sequencing to analyze gut bacterial communities of six populations of two bark beetle species in Mexico, identifying a core group of bacteria and their metabolic pathways.
- Findings indicate that while the bacterial community varies by location, the metabolic pathways are consistent, suggesting stability across geographic areas despite differences in bacterial composition.
The thermophilic biomass-degrading fungus Thielavia terrestris Co3Bag1 produces a hyperthermophilic and thermostable β-1,4-xylanase with exo- and endo-activity.
Yolanda García-Huante Maribel Cayetano-Cruz Alejandro Santiago-Hernández Claudia Cano-Ramírez Rodolfo Marsch-Moreno
Extremophiles
January 2017
Article Synopsis
- A thermostable xylanase enzyme (TtXynA) was extracted from T. terrestris Co3Bag1 and exhibits optimal activity at 85 °C and pH 5.5.
- TtXynA retains over 90% activity across a pH range of 4.5-10 and has a long half-life of 23.1 days at 65 °C, demonstrating high thermal stability.
- This enzyme displays strong activity on beechwood xylan, producing various sugars, and is the first reported hyperthermophilic xylanase from T. terrestris, indicating potential for use in high-temperature biotechnological processes like bioethanol production.
Article Synopsis
- * The method involves separating enzyme activities from various sources using a polyacrylamide gel, followed by enzyme refolding and visualization for MW estimation with Coomassie blue and Congo red staining.
- * This technique could be beneficial for screening enzymes, identifying polypeptides, and monitoring the production of cellulases and xylanases in bacteria and fungi.
Expression, purification and characterization of an endoglucanase from Serratia proteamaculans CDBB-1961, isolated from the gut of Dendroctonus adjunctus (Coleoptera: Scolytinae).
Claudia Cano-Ramírez Alejandro Santiago-Hernández Flor Nohemí Rivera-Orduña Yolanda García-Huante Gerardo Zúñiga
AMB Express
December 2016
Article Synopsis
- Serratia proteamaculans CDBB-1961, a gut symbiont of the roundheaded pine beetle, shows strong ability to break down cellulose, particularly using carboxymethyl cellulose as a carbon source.
- The identification of a specific gene, spr cel8A, encoding an endoglucanase enzyme (Spr Cel8A) indicates a high similarity to other known endoglucanases, while demonstrating optimal activity at pH 7.0 and 40 °C.
- The study reveals that Spr Cel8A can produce cellobiose and oligosaccharides, contributing to the understanding of S. proteamaculans' role in the beetle's gut, marking the
Article Synopsis
- Bark beetles, particularly the red turpentine beetle (RTB), are significant contributors to the death of woody plants worldwide, using specific chemical cues from trees to identify suitable hosts for colonization.
- This study identified and analyzed eleven cytochrome P450 genes in the antennae and gut of RTB that are likely involved in metabolizing monoterpenes, which are volatile compounds from pine trees that serve as kairomones for the beetles.
- The research also found that the expression of these genes varies between male and female RTBs and is influenced by the type of monoterpene and the duration of exposure, suggesting a role in detoxifying these compounds during tree colonization.
Article Synopsis
- Bark beetles use cytochrome P450 enzymes to oxidize defensive compounds in their host trees, both to detoxify them and to create components for their pheromones.
- Research identified ten new CYP genes in the pine beetle Dendroctonus rhizophagus, found in the gut and antennae, that respond to specific host tree compounds.
- The study showed that CYP gene expression varies by sex and is affected by time and exposure to host monoterpenes, indicating their role in detoxifying these chemicals.
Article Synopsis
- - The bark beetle Dendroctonus rhizophagus, native to northwestern Mexico, targets immature pines under 3 meters tall and its chemical ecology has been studied for the first time to understand its effects on forest regeneration.
- - Researchers used techniques to analyze how D. rhizophagus responds to various volatile compounds from their host Pinus arizonica and from beetles themselves; both sexes showed sensitivity to several monoterpenes and identified myrtenol and trans-verbenol as the most prevalent volatiles.
- - Field experiments revealed that 3-carene significantly attracts D. rhizophagus when combined with other monoterpenes, while beetle-associated oxygenated monoterpenes did
Article Synopsis
- * The method targets a specific copper-binding sequence of laccase genes and can effectively quantify these fungi in different culture setups by analyzing DNA fragments of varying sizes.
- * This protocol can also detect a third fungus, Phanerochaete chrysosporium, and has potential applications for monitoring and improving fungal cultures in wastewater treatment projects.