Specific cultivation-independent enumeration of viable cells in probiotic products using a combination of fluorescence hybridization and flow cytometry.

This study introduces an optimized integration of flow cytometry and fluorescence hybridization (Flow-FISH) as an approach for the specific enumeration of gram-positive bacteria in probiotic products, overcoming the limitations of conventional methods. The enhanced Flow-FISH technique synergizes the rapid and automated capabilities of flow cytometry with the high specificity of FISH, facilitating the differentiation of viable cells at the species level within probiotic blends. By analyzing lyophilized samples of , , and subsp.

Symbioflor2 Genotypes Enhance Ileal and Colonic Gene Expression Associated with Mucosal Defense in Gnotobiotic Mice.

Symbioflor2 is a probiotic product composed of six genotypes, which has a beneficial effect on irritable bowel syndrome. Our objective was to understand the individual impact of each of the six genotypes on the host, together with the combined impact of the six in the compound Symbioflor2. Gnotobiotic mice were mono-associated with one of the six genotypes or associated with the compound product.

Evaluation of the applicability of the aquatic ascomycete sp. UHH 5-1-03 for the removal of pharmaceutically active compounds from municipal wastewaters using membrane bioreactors.

Membrane bioreactors (MBRs) augmented with terrestrial white-rot basidiomycetes have already been tested for the removal of pharmaceutically active compounds (PhACs) from wastewaters. Within the present study, an aquatic ascomycete ( sp.) was initially demonstrated to efficiently remove several PhACs at their real environmental trace concentrations from nonsterile municipal wastewater on a laboratory scale.

A Review of Research Conducted with Probiotic Marketed as Symbioflor.

This review article summarizes the scientific literature that is currently available about a probiotic that is known under the name Symbioflor . The probiotic is marketed for human use and has been subjected to over 20 years of scientific research. As is presented here, the available literature not only contains multiple works to investigate and analyse the probiotic activity of this , but also describes a variety of other research experiments, dealing with a surprising and interesting range of subjects.

Virulence genes in a probiotic E. coli product with a recorded long history of safe use.

The probiotic product Symbioflor2 (DSM 17252) is a bacterial concentrate of six different Escherichia coli genotypes, whose complete genome sequences are compared here, between each other as well as to other E. coli genomes. The genome sequences of Symbioflor2 E.

Lab scale experiments using a submerged MBR under thermophilic aerobic conditions for the treatment of paper mill deinking wastewater.

This paper describes the results of laboratory experiments using a thermophilic aerobic MBR (TMBR) at 50 °C. An innovative use of submerged flat-sheet MBR modules to treat circuit wastewater from the paper industry was studied. Two experiments were conducted with a flux of 8-13 L/m(2)/h without chemical membrane cleaning.

Development and validation of a FISH-based method for the detection and quantification of E. coli and coliform bacteria in water samples.

Monitoring of microbiological contaminants in water supplies requires fast and sensitive methods for the specific detection of indicator organisms or pathogens. We developed a protocol for the simultaneous detection of E. coli and coliform bacteria based on the Fluorescence in situ Hybridization (FISH) technology.

Monitoring structure and activity of nitrifying bacterial biofilm in an automatic biodetector of water toxicity.

Automatic biodetector of water toxicity is a biosensor based on monitoring of catalytic activity of the nitrifying bacteria. To create a standardized biosensing system, development of the biofilm must be characterized to determine the prerequisites for its biological (biocatalytic) stability. In this paper, growth of biofilm comprising ammonium-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in the open cellular polyurethane material polyurethane sponge bioreactor has been investigated.

Optimization of conditions for profiling bacterial populations in food by culture-independent methods.

In this study we used culture-independent methods to profile bacterial populations in food products. Denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH) were employed in order to identify bacterial species without the need of isolation and biochemical identification. The protocols used to extract the DNA, subsequently subjected to PCR amplification for DGGE, as well as the hybridization procedure for FISH, were optimised.

Filamentous Alphaproteobacteria associated with bulking in industrial wastewater treatment plants.

The phylogeny and distribution of filamentous Alphaproteobacteria, morphologically similar to "Nostocoida limicola" and Eikelboom Type 021N that cause the solids separation problem of bulking in industrial activated sludge plants is described here. A combination of culture-dependent and culture-independent molecular methods has characterized 5 novel species. 16S rRNA targeted oligonucleotide probes were designed for their in situ identification by fluorescence in situ hybridisation (FISH) and used to monitor their presence in 86 WWTPs treating different industrial effluents in four European countries.

Fluorescence based rRNA sensor systems for detection of whole cells of Saccharomonospora spp. and Thermoactinomyces spp.

Airborne thermophilic actinomycetes (TPAs) are a growing hygienic challenge in different occupational situations e.g. large scale composting.