"A Man Has to Show That He Is a Man From a Young Age": Intimate Partner Violence for Young Male University Students in Brazil.

Considering that men today face social and cultural pressures to behave in certain ways, the objective of this study was to analyze the construction of Intimate Partner Violence (IPV) from the perspective of university students who identify themselves as male in a developing country. This is a qualitative study carried out through semi-structured interviews, with 15 students from the state of São Paulo, Brazil, from May to July 2021. The data were analyzed thematically and anchored in the ecological framework of understanding violence.

Real-time investigation of mannosyltransferase function of a Xylella fastidiosa recombinant GumH protein using QCM-D.

Xylella fastidiosa is a gram-negative bacterium that causes serious diseases in economically important crops, including grapevine, coffee, and citrus fruits. X. fastidiosa colonizes the xylem vessels of the infected plants, thereby blocking water and nutrient transport.

Overexpression, purification, biochemical characterization, and molecular modeling of recombinant GDP-mannosyltransferase (GumH) from Xylella fastidiosa.

The GumH enzyme from Xylella fastidiosa catalyzes the transfer reaction of a mannose from GDP-mannose to the carrier lipid cellobiose-pyrophosphate-polyprenol (Glc(2)-PP-Lip), an intermediary in the reaction for the synthesis of the exopolysaccharide (EPS) fastidian gum. The gumH gene was subcloned in the pMal-c2x vector, allowing the expression of the GumH-MBP fusion protein. Various attempts were made to obtain protein with the necessary degree of purity for crystallographic studies but the yield was very low.