Publications by authors named "Claude Celati"
Centrioles are components of the centrosome, which is present in most eukaryotic cells (from protozoa to mammals). They organize the microtubule skeleton during interphase and the mitotic spindle during cell division. In ciliate cells, centrioles form basal bodies that are involved in cellular motility.
View Article and Find Full Text PDFA comprehensive model of how the centrosome organises the microtubule network in animal cells has not yet been elucidated. Here we show that the centrosomal large CAP-Gly protein CAP350 is not only present at the centrosome, but is also present as numerous dots in the pericentrosomal area. Using in vitro and in vivo expression of partial constructs, we demonstrated that CAP350 binds microtubules through an N-terminal basic region rather than through its CAP-Gly domain.
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- Loss of normal primary cilia function is linked to diseases like polycystic kidney disease, indicating a connection between cilia and the cell cycle.
- IFT88/polaris, a key component of intraflagellar transport, stays associated with the centrosome throughout the cell cycle in a manner independent of microtubules and dynein.
- Overexpression of IFT88/polaris can block the G1-S transition and lead to cell death, while its depletion speeds up the cell cycle, suggesting it plays a crucial role in cell proliferation beyond its function in ciliogenesis.
The small Ran GTPase, a key regulator of nucleocytoplasmic transport, is also involved in microtubule assembly and nuclear membrane formation. Herein, we show by immunofluorescence, immunoelectron microscopy, and biochemical analysis that a fraction of Ran is tightly associated with the centrosome throughout the cell cycle. Ran interaction with the centrosome is mediated by the centrosomal matrix A kinase anchoring protein (AKAP450).
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