X-ray crystallographic structure of BshB, the zinc-dependent deacetylase involved in bacillithiol biosynthesis.

Many gram-positive bacteria produce bacillithiol to aid in the maintenance of redox homeostasis and degradation of toxic compounds, including the antibiotic fosfomycin. Bacillithiol is produced via a three-enzyme pathway that includes the action of the zinc-dependent deacetylase BshB. Previous studies identified conserved aspartate and histidine residues within the active site that are involved in metal binding and catalysis, but the enzymatic mechanism is not fully understood.

Sustained high levels of physical activity lead to improved performance among "Race Across the USA" athletes.

Objective: To investigate physiological and performance adaptations associated with extremely high daily sustained physical activity levels, we followed six runners participating in the 2015 Race Across the USA. Participants completed over 42.2 km a day for 140 days, covering nearly 5,000 km.