Design, Synthesis, and Evaluation of Cephamycin-Based Antisporulation Agents targeting .

With the aim of discovering small molecule inhibitors of the sporulation process in , we prepared a series of C-7 α-(4-substituted-1-1,2,3-triazol-1-yl)acetamide analogues of cefotetan, a known inhibitor of the sporulation-specific protein target SpoVD. These analogues were evaluated using both binding assays with SpoVD and antisporulation assays against . Further design concepts were aided utilizing the predicted docking scores (DS) using both AlphaFold (AF) models, and a crystal structure of the SpoVD protein (PDB 7RCZ).