Objective: to describe the knowledge, attitude and practice of vaccinators toward childhood vaccination.
Methods: this is a descriptive census study, conducted in 2015 with vaccinators from vaccination rooms of the urban area of Teresina, Piauí, Brazil; data were collected through a questionnaire.
Results: 70 vaccinators were interviewed; most answers related to knowledge and attitude of the vaccinators toward childhood vaccination were considered inadequate (n=58 and n=39, respectively); with regard to practice, there were 43 adequate answers; there was no statistically significant association between the variable knowledge and the variables attitude and practice.