Oxy-PAHs: occurrence in the environment and potential genotoxic/mutagenic risk assessment for human health.

Structurally modified polycyclic aromatic hydrocarbons (PAHs) such as nitrated PAHs (nitro-PAHs) and oxygenated PAHs (oxy-PAHs) can be incriminated in the total toxicity of polycyclic aromatic compounds (PACs) fraction in the environment. Compared to nitro-PAHs, oxy-PAHs have been poorly studied. Oxy-PAHs covers compounds with different moieties such as polycyclic aromatic ketones (PAKs) and polycyclic aromatic quinones (PAQs).

High-resolution magic angle spinning description of the interaction states and their kinetics among basic solutes and functionalized silica materials.

Modeling of the interaction is crucial to understanding and predicting chromatography. However, the complexity and variety of the grafted motifs render the creation of an accurate model overwhelmingly challenging, so that most often the classification of column separation properties is described by monitoring the retention times of carefully selected control molecules. We analyzed here the characteristics of the interplay of compounds of basic nature by (1)H HRMAS NMR, which provide relevant descriptors for products with pharmaceutical properties, with chromatographic phases for Reversed Phase Liquid Chromatography.

Metabolic expressivity of human genetic variants: NMR metabotyping of MEN1 pathogenic mutants.

Functional consequences of mutations in predisposition genes for familial cancer syndromes remain often elusive, especially when the corresponding gene products play pleiotropic functions and interact with numerous partners. Understanding the consequences of these genetic alterations requires access to their functional effects at the phenotypic level. Nuclear magnetic resonance (NMR) has emerged as a promising functional genomics probe, through its ability to monitor the consequences of genetic variations at the biochemical level.

Chromatographic-nuclear magnetic resonance can provide a prediction of high-pressure liquid chromatography shape selectivity tests.

NMR diffusometry has been recently demonstrated as a means of investigating the mobility variations of solutes induced by chromatographic phases (under the acronym chromatographic-NMR). Particularly, a given compound has its average diffusivity reduced proportionally to its affinity towards the solid. In this work we propose the first comparison of chromatographic-NMR and tests for assessment of column performance, to investigate to what measure the novel approach could provide an assay of the outcome of a given stationary phase without the need of packing the relative column.

Synthesis and study of a molecularly imprinted polymer for specific solid-phase extraction of vinflunine and its metabolite from biological fluids.

A molecularly imprinted polymer (MIP) was synthesized in order to specifically extract vinflunine, an anticancer agent, and its metabolite (4-O-deacetylvinflunine) from bovine plasma and artificial urine by solid-phase extraction (SPE). Vinorelbine, a non-fluorinated analogue of vinflunine, was selected as a template for MIP synthesis. The selectivity of MIP versus the template (vinorelbine) and other alkaloids (catharanthine, vinblastine, vincristine, vinflunine and 4-O-deacetylvinflunine) was shown by a SPE protocol carried out with non-aqueous samples.