Publications by authors named "Claire Engstrom"
Genetically modified, induced pluripotent stem cells (iPSCs) offer a promising allogeneic source for the generation of functionally enhanced, chimeric antigen receptor (CAR) T cells. However, the signaling of CARs during early T cell development and the removal of the endogenous T cell receptor required to prevent alloreactivity pose significant challenges to the production of mature conventional CAR T cells from iPSCs. Here, we show that TCR-null, CD8αβ CAR T cells can be efficiently generated from iPSCs by engineering stage-specific onset of CAR expression and signaling to both permit conventional T cell development and to induce efficient positive selection.
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- - Pluripotent stem cells (PSCs) are being explored as a source for off-the-shelf T cell immunotherapies, but their differentiation into mature T cells can lead to complications if not properly managed.
- - Researchers successfully generated mature T cells from genetically edited PSCs that lack specific T cell receptors (TCRs) and class I major histocompatibility complexes, using a combination of human and murine cells for T cell maturation.
- - The study found that these edited T cells demonstrated significantly improved tumor control in mouse models compared to those with intact TCRs, suggesting a promising approach for enhancing T cell immunotherapies derived from PSCs.