Mild obesity does not affect perinatal outcome in gestational carrier cycles.

Study Question: Does BMI of gestational carriers (GCs) affect perinatal outcomes after embryo transfer?

Summary Answer: Overweight and class I obesity in GCs does not affect the rate of good perinatal outcomes.

What Is Known Already: The use of GCs is increasing, but uniform guidance regarding optimal BMI for GCs is lacking. Women with obesity who conceive without fertility treatment or through autologous or donor in vitro fertilization are at higher risk of adverse maternal and fetal outcomes, but data on obesity in GCs are very limited.

Obstructed Hemivagina and Renal Anomalies in Patients with and without Anorectal Malformations.

Study Objective: To compare the anatomic variation between patients with a diagnosis of an obstructed hemivagina with an anorectal malformation (ARM) and those without an ARM.

Methods: This was a retrospective chart review conducted at a single tertiary children's hospital. Patients with an obstructed hemivagina seen from 2004 to 2019 were included.

Use of Hormone Therapy in Women with Early Menopause and Premature Ovarian Insufficiency.

Women with early menopause or primary ovarian insufficiency (POI) experience a menopausal state a decade or more earlier than their peers. The health consequences for POI are vast and varied with detrimental effects seen on neurological, psychological, bone, and cardiovascular systems. The risk profile of POI patients requires special attention, as they differ from a typical menopausal population.

The Polyphenol-Rich Extract from an Endemic Medicinal Plant from Reunion Island, Inhibits the Early Stages of Dengue and Zika Virus Infection.

The recent emergence and re-emergence of viral infections transmitted by vectors, such as the Zika virus (ZIKV) and Dengue virus (DENV), is a cause for international concern. These highly pathogenic arboviruses represent a serious health burden in tropical and subtropical areas of the world. Despite the high morbidity and mortality associated with these viral infections, antiviral therapies are missing.

Extract from Aphloia theiformis, an edible indigenous plant from Reunion Island, impairs Zika virus attachment to the host cell surface.

The mosquito-borne Zika virus (ZIKV) belongs to the flavivirus genus of the Flaviviridae family. Contemporary epidemic strains of ZIKV are associated with congenital malformations in infants, including microcephaly, as well as Guillain-Barré syndrome in adults. A risk of human-to-human transmission of ZIKV is also well documented.

A robust method for the rapid generation of recombinant Zika virus expressing the GFP reporter gene.

Zika virus (ZIKV) infection is a major public health problem with severe human congenital and neurological anomalies. The screening of anti-ZIKV compounds and neutralizing antibodies needs reliable and rapid virus-based assays. Here, we described a convenient method leading to the rapid production of molecular clones of ZIKV.

Orexin gene therapy restores the timing and maintenance of wakefulness in narcoleptic mice.

Study Objectives: Narcolepsy is caused by selective loss of the orexin/hypocretin-producing neurons of the hypothalamus. For patients with narcolepsy, chronic sleepiness is often the most disabling symptom, but current therapies rarely normalize alertness and do not address the underlying orexin deficiency. We hypothesized that the sleepiness of narcolepsy would substantially improve if orexin signaling were restored in specific brain regions at appropriate times of day.

Expression of endothelin and ETB receptors in the megakaryoblastic MEG-01 cell line.

The presence of endothelin (ET) receptors and the nature of the subtype and expression of ET were investigated in the human megakaryoblastic cell line MEG-01. By the RT-PCR procedure, we have shown that both ETA and ETB receptor subtype mRNAs are expressed in the cells. However, binding experiments have shown that the selective ETB receptor antagonist BQ788, but not the selective ETA receptor antagonist BQ123, competes with the specific binding of [125I]ET-1.

Effects of oxygen-free radicals on proliferation kinetics of cultured rabbit articular chondrocytes.

It can be postulated that among the factors implicated in cartilaginous lesions, oxygen-derived free radicals seem to have a prominent part. To investigate this hypothesis, rabbit articular chondrocyte cultures have been exposed to oxygen-derived reactive species generated by the hypoxanthine-xanthine oxydase system. We observed a dose-dependent decrease of cellular growth.

Cytotoxicity of D-penicillamine in association with several heavy metals against cultured rabbit articular chondrocytes.

The potentiation or antagonistic effects of Cu, Hg, Pb and Cd salts in the presence of a long-acting anti-rheumatic drug, D-penicillamine (D.P.) were studied on cultured chondrocytes.

Hydroxyurea-induced mitotic synchronization in Allium sativum root meristems.

The synchronized divisions following a treatment with hydroxyurea (HU) - an inhibitor of DNA synthesis - were studied in root meristems of Allium sativum using two methods: autoradiography of median sections and morphological labeling with a cytokinesis inhibitor. It is shown that the second wave of mitoses is heterogeneous: it is composed mostly of cells which have been synchronized in the S phase by the HU treatment, of cells coming from the quiescent center stimulated to enter DNA synthesis and of cells which were not blocked by the 23 h HU treatment (slow cycling cells). It is also shown that the cell cycle following the first synchronized division is considerably shortened by the synchronization procedure.

[Cytotoxicity of cadmium : study on root meristems of Allium sativum L].

Cadmium nitrate, acetate and sulphate cause death of root meristems of Allium sativum at 5.10(-7) Mol/ml concentration for the two first ones and 10(-7) Mol/ml for the last one. Lower concentrations do not induce chromosomal aberrations.

[Cytotoxicity of aurintricarboxylic acid: inhibition of cell proliferation and of protein synthesis in Allium sativum L. root meristems].

The inhibitory action of aurine-tricarboxylic acid (ATA) on protein synthesis, known in vitro, has been checked in vivo in Allium sativum L. root meristems. Parallel to this inhibition, ATA acts on cell proliferation, on the one hand by preventing the entering of cells into prophase and, on the other hand, by disturbing the process of mitosis.