Publications by authors named "Citra Praditi"
Article Synopsis
- Glioblastoma, an aggressive brain cancer, is linked to low oxygen levels (hypoxia) and poor patient survival, driven by the activity of a transcription factor called hypoxia-inducible factor (HIF).
- HIF-hydroxylases, which require vitamin C (ascorbate) to function, are crucial for regulating HIF and the hypoxic pathway; a decrease in these enzymes leads to increased cancer progression.
- The study showed that glioblastoma cells use the sodium-dependent vitamin C transporter-2 (SVCT2) to accumulate ascorbate, and knocking out this transporter significantly reduced ascorbate levels and limited its ability to suppress the hypoxic pathway, suggesting that boosting ascorbate levels could
Ascorbate plays a vital role as a co-factor for a superfamily of enzymes, the 2-oxoglutarate dependent dioxygenases (2-OGDDs), which govern numerous pathways in cancer progression, including the hypoxic response and the epigenetic regulation of gene transcription. Ascorbate uptake into most cells is through active transport by the sodium-dependent vitamin C transporter 2 (SVCT2). The aims of this study were to determine the kinetics of ascorbate uptake and retention by breast cancer cell lines under various oxygen conditions, and to investigate the role of SVCT2 in mediating ascorbate uptake and intracellular trafficking.
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