Mechanism of HS Formation from the Metabolism of Anetholedithiolethione and Anetholedithiolone by Rat Liver Microsomes.

The drug anetholedithiolethione (ADT) and its analogs have been extensively used as HS donors. However, the mechanism of HS formation from ADT under biologic conditions remains almost completely unknown. This article shows that only small amounts of HS are formed during incubation of ADT and of its metabolite anetholedithiolone (ADO) with rat liver cytosol or with rat liver microsomes (RLM) in the absence of NADPH, indicating that HS formation under these conditions is of hydrolytic origin only to a minor extent.

Quantitative analysis of the tumor suppressor dendrogenin A using liquid chromatography tandem mass spectrometry.

Dendrogenin A (DDA) was recently identified as a mammalian cholesterol metabolite that displays tumor suppressor and neurostimulating properties at low doses. In breast tumors, DDA levels were found to be decreased compared to normal tissues, evidencing a metabolic deregulation of DDA production in cancers. DDA is an amino-oxysterol that contains three protonatable nitrogen atoms.