Serum albumin binding knob domains engineered within a V framework III bispecific antibody format and as chimeric peptides.

Background: Serum albumin binding is an established mechanism to extend the serum half-life of antibody fragments and peptides. The cysteine rich knob domains, isolated from bovine antibody ultralong CDRH3, are the smallest single chain antibody fragments described to date and versatile tools for protein engineering.

Methods: Here, we used phage display of bovine immune material to derive knob domains against human and rodent serum albumins.

Monoclonal Autoantibody Against a Cryptic Epitope on Tissue-Adherent Low-Density Lipoprotein for Molecular Imaging in Atherosclerosis.

Background: Antibody-based constructs for molecular imaging and therapeutic delivery provide promising opportunities for the diagnosis and treatment of atherosclerosis.

Objectives: The authors aimed to generate and characterize immunoglobulin (Ig)G monoclonal autoantibodies in atherosclerosis for targeting of novel molecular determinants.

Methods: The authors created hybridomas from an unimmunized low-density lipoprotein (LDL) receptor-deficient (Ldlr) mouse and selected an IgG2b isotype autoantibody, LO9, for further characterization.