Identification of four novel HLA alleles: HLA-B*27:265, -B*35:569, -DRB1*08:117 and -DPB1*1435:01.

Identification of four new HLA alleles (B*27:265, B*35:569, DRB1*08:117, and DPB1*1435:01) in Brazilian bone marrow donors.

Interlocus gene conversion: Identification of HLA-A*23:128 in a Brazilian bone marrow donor.

Identification of novel HLA-A*23:128 allele generated by interlocus gene conversion in Brazilian bone marrow donor.

The novel HLA-DRB1*03:178, -DRB1*03:179, and -DRB1*11:276 alleles identified in a healthy Brazilian individuals.

The novel HLA-DRB1*03:178, -DRB1*03:179, -DRB1*11:276 alleles were identified in healthy Brazilian individuals.

Five novel HLA-A, -B, and -C alleles identified in Brazilian individuals by next-generation sequencing.

The novel HLA-A*11:379, B*45:01:11, B*15:571, B*57:137, C*07:893 alleles were identified in Brazilian individuals.

Characterization of 15 novel HLA alleles by next generation sequencing in Brazilian individuals.

Fifteen novel HLA alleles in the HLA-A, -B, -C, DRB1, and DQB1 loci were described.

Next-generation sequencing of HLA: validation and identification of new polymorphisms in a Brazilian population.

Background: Next-generation sequencing (NGS) is the most modern sequencing technique that has revolutionized HLA typing, providing high-resolution results with low ambiguity rates. This study aimed to show the experiences and challenges of an HLA laboratory in the validation process of the NGS methodology for HLA typing and show the use of this method for the study of HLA genetic diversity.

Methods: We used 115 samples that comprised a comprehensive testing panel for validation of the NGS methodology using the AllType kit (One Lambda, Canoga Park, California) on the Ion Torrent S5 NGS platform.