Low specificity of spp. ELITe MGB assay, and potential risks in management of invasive aspergillosis.

Objectives: In recent years, commercial molecular tools for diagnosis of invasive aspergillosis have emerged, requiring evaluation to ensure quality. Here we assessed the specificity of spp.-ELITe MGB Assay a commercial assay tergeting 18S gene of spp.

How to choose the right real-time RT-PCR primer sets for the SARS-CoV-2 genome detection?

Objectives: The SARS-CoV-2 pandemic has created an unprecedented need for rapid large-scale diagnostic testing to prompt clinical and public health interventions. Currently, several quantitative reverse-transcription polymerase chain reaction (RT-qPCR) assays recommended by the World Health Organization are being used by clinical and public health laboratories and typically target regions of the RNA-dependent RNA polymerase (RdRp), envelope (E) and nucleocapsid (N) coding region. However, it is currently unclear if results from different tests are comparable.