Impact of abiotic stressors on nutrient removal and rhizomicrobiome composition in floating treatment wetland with Equisetum hyemale.

Floating treatment wetlands (FTW) are receiving growing interest as a phyto-technology. However, there are significant research gaps regarding the actual role of plant species and plant-microbiome interactions. In this study, the nutrient uptake of Equisetum hyemale was examined in FTW microcosms under the influence of abiotic stressors: As (3 mg/L) and Pb (3 mg/L) as well as Cl (300 and 800 mg/L) in reference to a control during a short screening experiment.

Enhancing nitrogen removal in the partial denitrification/anammox processes for SO - Rich wastewater treatment: Insights into autotrophic and mixotrophic strategies.

The investigation of partial denitrification/anammox (PD/anammox) processes was conducted under autotrophic (N-S cycle) and mixotrophic (N-S-C cycle) conditions over 180 days. Key findings revealed the remarkable capability of SO-dependent systems to produce NO effectively, supporting anaerobic NH oxidation. Additionally, SO served as an additional electron acceptor in sulfate reduction ammonium oxidation (SRAO).

Fine-Tuning of DADA2 Parameters for Multiregional Metabarcoding Analysis of 16S rRNA Genes from Activated Sludge and Comparison of Taxonomy Classification Power and Taxonomy Databases.

Taxonomic classification using metabarcoding is a commonly used method in microbiological studies of environmental samples and during monitoring of biotechnological processes. However, it is difficult to compare results from different laboratories, due to the variety of bioinformatics tools that have been developed and used for data analysis. This problem is compounded by different choices regarding which variable region of the gene and which database is used for taxonomic identification.

Comparative structural and functional analysis of the glycine-rich regions of Class A and B J-domain protein cochaperones of Hsp70.

J-domain proteins are critical Hsp70 co-chaperones. A and B types have a poorly understood glycine-rich region (G) adjacent to their N-terminal J-domain (J). We analyzed the ability of J/G segments of yeast Class B Sis1 and a suppressor variant of Class A, Ydj1, to rescue the inviability of sis1-∆.

The role of the combined nitrogen-sulfur-carbon cycles for efficient performance of anammox-based systems.

The combined anammox/mixotrophic denitrification process was conducted in two granular sequencing batch reactors (SBRs) during a 200-day operation. Both reactors were fed with synthetic medium, but SBR2 was enriched with additional sulfate (SO) which influenced sulfate reduction ammonium oxidation (SRAO) and heterotrophic reduction of SO by sulfate reducing bacteria. It was hypothesized that the addition of SO could positively impact the removal rates of N-S-C compounds.

The Hsp70 and JDP proteins: Structure-function perspective on molecular chaperone activity.

Proteins are the most structurally diverse cellular biomolecules that act as molecular machines driving essential activities of all living organisms. To be functional, most of the proteins need to fold into a specific three-dimensional structure, which on one hand should be stable enough to oppose disruptive conditions and on the other hand flexible enough to allow conformational dynamics necessary for their biological functions. This compromise between stability and dynamics makes proteins susceptible to stress-induced misfolding and aggregation.

Analysis of Reconstituted Tripartite Complex Supports Avidity-based Recruitment of Hsp70 by Substrate Bound J-domain Protein.

Hsp70 are ubiquitous, versatile molecular chaperones that cyclically interact with substrate protein(s). The initial step requires synergistic interaction of a substrate and a J-domain protein (JDP) cochaperone, via its J-domain, with Hsp70 to stimulate hydrolysis of its bound ATP. This hydrolysis drives conformational changes in Hsp70 that stabilize substrate binding.

Integration of the sulfate reduction and anammox processes for enhancing sustainable nitrogen removal in granular sludge reactors.

The Anammox and Sulfate Reduction Ammonium Oxidation processes were compared in two granular sequencing batch reactors operated for 160 days under anammox conditions. It was hypothesized that increasing the concentration of SO may positively influence the rate of N removal under anaerobic conditions and it was tested whether SO reduction and anammox occur independently or are related to each other. The cooperation of N-S cycles by increasing the concentration of influent SO to 952 mg S/L in the second reactor, a higher ammonium utilization rate and sulfate utilization rate was achieved compared to the first reactor, i.

The coexistence and competition of canonical and comammox nitrite oxidizing bacteria in a nitrifying activated sludge system - Experimental observations and simulation studies.

The second step of nitrification can be mediated by nitrite oxidizing bacteria (NOB), i.e. Nitrospira and Nitrobacter, with different characteristics in terms of the r/K theory.

Chemical Structure of Stabilizing Layers of Negatively Charged Silver Nanoparticles as an Effector of Shifts in Soil Bacterial Microbiome under Short-Term Exposure.

In this work, we have assessed the exposure of soil bacteria from potato monoculture to three types of silver nanoparticles (AgNPs) as well as silver ions (Ag ions) delivered in the form of silver nitrate and a commercially available fungicide. The diversity of the soil microbial community, enzymatic activity, and carbon source utilization were evaluated. It was found that only the fungicide significantly limited the abundance and activity of soil bacteria.

The Resistome of ESKAPEE Pathogens in Untreated and Treated Wastewater: A Polish Case Study.

The aim of this study was to quantify ESKAPEE bacteria, genes encoding resistance to antibiotics targeting this group of pathogens, as well as integrase genes in municipal wastewater and river water. Environmental DNA was extracted from the collected samples and used in deep sequencing with the Illumina TruSeq kit. The abundance of bacterial genera and species belonging to the ESKAPEE group, 400 ARGs associated with this microbial group, and three classes of integrase genes were determined.

Leading as Servant in Times of Crisis.

Using servant leadership as a framework, leaders within a school of nursing guided faculty during the COVID-19 pandemic and called them to address social injustice in the summer of 2020. A model was developed to sustain growth and build resilience of students, faculty, and staff. Influenced by external and internal drivers that stimulated the need for change, strategies were implemented based on core principles of servant leadership.

Development of microbial communities in biofilm and activated sludge in a hybrid reactor.

Microorganisms play a key role in biological wastewater treatment. The form in which biomass develops determines the efficiency and mechanisms of organic compound conversion, due to different conditions in various microbial structures. However, the results of studies comparing the microbial communities in biofilm and activated sludge have often conflicted.

Rearrangements of the nitrifiers population in an activated sludge system under decreasing solids retention times.

Due to the key role of nitrite in novel nitrogen removal systems, nitrite oxidizing bacteria (NOB) have been receiving increasing attention. In this study, the coexistence and interactions of nitrifying bacteria were explored at decreasing solids retention times (SRTs). Four 5-week washout experiments were carried out in laboratory-scale (V = 10 L) sequencing batch reactors (SBRs) with mixed liquor from two full-scale activated sludge systems (continuous flow vs SBR).

Polyhydroxyalkanoates production from short and medium chain carboxylic acids by Paracoccus homiensis.

The aim of this study was to evaluate an effect of short and medium chain carboxylic acids (CAs) rich stream derived from acidogenic mixed culture fermentation of acid whey on polyhydroxyalkanoates (PHAs) synthesis by Paracoccus homiensis and compare it with the impact of individual synthetic CAs. The obtained results confirmed that the analyzed bacterium is able to metabolize synthetic CAs as the only carbon sources in the growth medium with maximum PHAs production yields of 26% of cell dry mass (CDM). The replacement of the individual CAs by a CAs-rich residual stream was found to be beneficial for the Paracoccus homiensis growth.

Polyethylene, polystyrene and lignocellulose wastes as mealworm (Tenebrio molitor L.) diets and their impact on the breeding condition, biometric parameters, metabolism, and digestive microbiome.

This study aimed to identify the extent to which a diet of oatmeal and polymers affects the development of mealworms, their microbiome, the biochemical activity of their digestive system, and their feed-metabolizing capacity. With a polystyrene diet, feed loss was most significant, as indicated by FTIR (Fourier-transform infrared spectroscopy) of frass, which showed that polystyrene was the only compound that was chemically modified. Compared to the control diet, mealworm larvae developed best on polyethylene regranulate (PE-reg), quickly transiting from one developmental stage to another with minor mass loss.

Characterization and phylogenetic analysis of the complete mitochondrial genome of the pathogenic fungus Ilyonectria destructans.

Ilyonectria destructans is a pathogenic fungus causing root rot and other symptoms on trees and many crops. This paper analyses the mitochondrial genome of I. destructans and compares it with other published Nectriaceae mitogenomes.

Neurodevelopment vs. the immune system: Complementary contributions of maternally-inherited gene transcripts and proteins to successful embryonic development in fish.

The aim of this study was to investigate the respective contribution of maternally-inherited mRNAs and proteins to egg molecular cargo and to its developmental competence in fish using pikeperch as a model. Our study provides novel insights into the understanding of type-specific roles of maternally-inherited molecules in fish. Here we show, for the first time, that transcripts and proteins have distinct, yet complementary, functions in the egg of teleost fish.

Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production.

Waste of industrial origin produced from synthetic materials are a serious threat to the natural environment. The ending resources of fossil raw materials and increasingly restrictive legal standards for the management of plastic waste have led to research on the use of biopolymers, which, due to their properties, may be an ecological alternative to currently used petrochemical polymers. Polyhydroxyalkanoates (PHAs) have gained much attention in recent years as the next generation of environmentally friendly materials.

Transcriptome Changes in KT2440 during Medium-Chain-Length Polyhydroxyalkanoate Synthesis Induced by Nitrogen Limitation.

's versatility and metabolic flexibility make it an ideal biotechnological platform for producing valuable chemicals, such as medium-chain-length polyhydroxyalkanoates (mcl-PHAs), which are considered the next generation bioplastics. This bacterium responds to environmental stimuli by rearranging its metabolism to improve its fitness and increase its chances of survival in harsh environments. Mcl-PHAs play an important role in central metabolism, serving as a reservoir of carbon and energy.

Assessment of diversity and composition of bacterial community in sludge treatment reed bed systems.

Due to their low emission of odours and lack of the need to apply additional chemical agents, sludge treatment reed beds (STRBs) constitute an economically feasible and eco-friendly approach to sewage sludge management. Correctly designed and operated STRBs ensure effective reduction of the dry matter content coupled with the mineralisation of organic compounds. Successful operation of STRBs relies on complex interactions between the plants and microorganisms responsible for the decomposition of organic matter and nutrient cycling.

Domestication modulates the expression of genes involved in neurogenesis in high-quality eggs of Sander lucioperca.

Pikeperch, Sander lucioperca, is a species of high interest to the aquaculture. The expansion of its production can only be achieved by furthering domestication level. However, the mechanisms driving the domestication process in finfishes are poorly understood.

Two-step mechanism of J-domain action in driving Hsp70 function.

J-domain proteins (JDPs), obligatory Hsp70 cochaperones, play critical roles in protein homeostasis. They promote key allosteric transitions that stabilize Hsp70 interaction with substrate polypeptides upon hydrolysis of its bound ATP. Although a recent crystal structure revealed the physical mode of interaction between a J-domain and an Hsp70, the structural and dynamic consequences of J-domain action once bound and how Hsp70s discriminate among its multiple JDP partners remain enigmatic.