Publications by authors named "Cidiane Graciele-Melo"
Visceral leishmaniasis (VL) is a zoonosis caused by the protozoa of the genus Leishmania. Among the species, L. infantum and/or L.
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- Diagnosing visceral leishmaniasis (VL) is difficult, prompting studies to evaluate various diagnostic tools, including polymerase chain reaction (PCR) for detecting Leishmania species in biological samples.
- This study compared conventional PCR and real-time PCR using the Sybr Green system, focusing on blood samples from 30 patients suspected of having VL, of whom 19 were confirmed by other methods.
- Results showed that both PCR techniques were equally effective in detecting the parasite's genome, even in patients without detectable antibodies, with 79% positivity in samples tested using both methods.