Variability of spectra of laser-induced fluorescence of colonic mucosa: its significance for fluorescence detection of colonic neoplasia.

To determine the extent of a natural variability of the spectra of the autofluorescence and its significance for a reproducibility of different approaches typically used in studies on fluorescence detection of colonic lesions. Two independent series of experiments have been conducted during three years in the same laboratory. Macroscopic tissue specimens obtained during operations of patients with colonic cancers were studied in vitro.

Fluorescence in situ detection of human cutaneous melanoma: study of diagnostic parameters of the method.

Multicenter study of the diagnostic parameters was conducted by three groups in Poland to determine if in situ fluorescence detection of human cutaneous melanoma based on digital imaging of spectrally resolved autofluorescence can be used as a tool for a preliminary selection of patients at increased risk of the disease. Fluorescence examinations were performed for 7228 pigmented lesions in 4079 subjects. Histopathologic examinations showed 56 cases of melanoma.

Detection of melanomas by digital imaging of spectrally resolved ultraviolet light-induced autofluorescence of human skin.

Digital images of autofluorescence excited with 366 nm Hg line were recorded in a narrow 475 nm band for 408 pigmented lesions of the skin (90 melanomas, 205 common melanocytic and dysplastic naevi, 113 lesions of different kinds) and analysed photometrically with respect to spatial distribution of intensity to differentiate between melanomas and other melanocytic lesions. Earlier reports describing patterns of intensity distributions characteristic for melanomas have not been confirmed in this study. However, our evaluations showed that an algorithm based on ratios of maximum intensity recorded outside the lesions and minimum intensity found within them, allows melanomas to be detected with a sensitivity of 82.

Ultraviolet laser-induced fluorescence of human stomach tissues: detection of cancer tissues by imaging techniques.

Background And Objective: The background for this work was several literature reports on applications of the fluorescence methods to detection and localization of human cancers. The objective of our study has been to investigate if such an approach could be applied for the detection of gastric cancers.

Study Design/materials And Methods: Our study was designed in such a way that spectrally resolved images of laser-induced fluorescence of human gastric mucosa were collected and assessed from a point of view of elaborating an algorithm allowing for a differentiation between malignant and premalignant lesions and areas of normal mucosa.

[Tissue spectroscopy. New generation of optical methods for cancer detection].

Recent developments in the field of new, non-invasive, sensitive methods of cancer detection based on measurements of autofluorescence of cells are discussed. Research oriented on a detection of human cancer has been carried on by several groups for last six years only but has already yielded important data pointing to a possibility of both in situ and in vitro detection of cancerous tissues in several human organs, especially lung, gynecological tract, skin and gastro-intestinal tract. At least two such methods have been currently subject to clinical tests.

Efficient polarization analyzer for Lyman(alpha) radiation.

A new efficient polarization analyzer for Lyman(alpha) radiation is described and compared with previously reported instruments.