Significant genetic differentiation of Gobiopterus lacustris, a newly recorded transparent goby in China.

In our recent survey, the transparent small Lacustrine goby, Gobiopterus lacustris had reported as the endemic species of Luzon, Philippines, was identified as an abundant species in mangroves of Leizhou Peninsula, China. Here, high diversity and significant differentiation of five sites of samples representing the west and east populations were revealed by mitochondrial DNA sequences. Five haplotypes of 56 cytochrome oxidase subunit I (Cox1) with the lengths of 623 base pairs (bp) have the high pairwise identity (>98.

Analysis of the complete mitochondrial genome sequence of .

The complete mitochondrial genome sequence of was obtained using PCR amplification and walking sequencing (GenBank accession no. JN_542716). The complete mitochondrial genome of was 15,665 bp long and showed significant AT bias (67% AT content, 33% GC content).

Morphological Characters and Transcriptome Profiles Associated with Black Skin and Red Skin in Crimson Snapper (Lutjanus erythropterus).

In this study, morphology observation and illumina sequencing were performed on two different coloration skins of crimson snapper (Lutjanus erythropterus), the black zone and the red zone. Three types of chromatophores, melanophores, iridophores and xanthophores, were organized in the skins. The main differences between the two colorations were in the amount and distribution of the three chromatophores.

Mitogenomes of genus Pristipomoides, Lutjanus and Pterocaesio confirm Caesionidae nests in Lutjanidae.

In this study, the complete mitochondrial genome of Goldbanded jobfish (Perciformes: Lutjanidae: Etelinae: Pristipomoides multidens) was sequenced using long-PCR amplification (Accession No.: KF430626). The mitochondrial genome is highly homologous to those of other teleosts.

The complete mitochondrial genome sequence of Trichiurus nanhaiensis (Perciformes: Trichiuridae).

The Naihai cutlassfish (Trichiurus nanhaiensis, Wang and Xu, 1992) is an important commercial fish species in South China Sea, while the taxonomy is still controversial. The 17,060 base pair mitochondrial genome sequence of T. nanhaiensis was determined, which shared the features with the other bony fishes for gene arrangement, nucleotide composition, and tRNA structures except for tRNA(pro) gene.

DNA barcoding South China Sea fishes.

We have determined 222 DNA barcode sequences of 95 fish species in 86 genera of 69 families from 15 orders. Fish were captured by trawl from two important fisheries regions in South China Sea: Spratly Islands (Nansha Islands) and Beibu Gulf. The average genetic distances between intraspecies were about 60-fold less than those of interspecies within different taxonomic levels, as Kimura two-parameter genetic distances averaged 17.

Phylogenetics inferred from mitogenome and control region of Silver Sillago, Sillago sihama.

Background And Aims: Sillago sihama is one of the commercially important fish species and the mitochondrial genome of S. sihama was determined.

Results: The mitogenome of S.

DNA barcoding, phylogenetic relationships and speciation of snappers (genus Lutjanus).

The phylogenetic relationships of 13 snapper species from the South China Sea have been established using the combined DNA sequences of three full-length mitochondrial genes (COI, COII and CYTB) and two partial nuclear genes (RAG1, RAG2). The 13 species (genus Lutjanus) were selected after DNA barcoding 72 individuals, representing 20 species. Our study suggests that although DNA barcoding aims to develop species identification systems, it may also be useful in the construction of phylogenies by aiding the selection of taxa.

[Screening and genetic diversity analysis of microsatellite markers in Chinese lobster (Panulirus stimpsoni)].

With the construction of a library of partial fractionated genomic DNA of Panulirus stimpsoni Hoehuis, the microsatellite sequences of P. stimpsoni were screened by PCR technique. Then, the genetic diversity was analyzed with the microsatellite markers.

[Suffocation point and diurnal metabolism pattern of Hemifusus tuba (Gmelin)].

The individuals of Hemifusus tuba (Gmelin) were divided into 3 groups, i. e., small [S, (6.

Phylogenetic relationships of South China Sea snappers (genus Lutjanus; family Lutjanidae) based on mitochondrial DNA sequences.

Phylogenetic relationships of intra- and interspecies were elucidated based on complete cytochrome b (cyt b) and cytochrome c oxidase subunit II (COII) gene sequences from 12 recognized species of genus Lutjanus Bloch in the South China Sea (SCS). Using the combined data set of consensus cyt b and COII gene sequences, interspecific relationships for all 12 recognized species in SCS were consistent with Allen's morphology-based identifications, with strong correlation between the molecular and morphological characteristics. Monophyly of eight species (L.

[Microsatellite analysis of genetic diversity and phylogenetic relationship of nine species of grouper in genus Epinephelus].

Thirteen microsatellite markers of Epinephelus awoara previously discovered by our lab were selected to analyze the genetic diversity and phylogenetic relationship of nine species of grouper (E. awoara, E. merra, E.

[Rapid isolation and characteristics analysis of microsatellites from Lutjanus russelli].

The (CA)n DNA sequences in Lutjanus russelli were isolated through PCR arrays, and the characteristics of the microsatellite were analyzed. DNA was extracted from a sample of Lutjanus russelli, and digested with Hae+Dra. The fragments were ligated to pUCm-T vector and transferred into DH5alpha to construct a genomic library.