Publications by authors named "Chutikarn Chitboonthavisuk"
Article Synopsis
- Understanding gene essentiality is key for linking genotype to phenotype, and PhageMaP is a new method that creates knockout libraries to examine this in bacteriophages.
- PhageMaP generates gene essentiality maps for various phages, revealing insights into gene function and conditional essentiality related to their hosts.
- The tool also helps identify phage genes affecting anti-phage defenses and allows for the engineering of more effective synthetic phages by transferring defense inhibitors.
Bacterial host factors regulate the infection cycle of bacteriophages. Except for some well-studied host factors (e.g.
View Article and Find Full Text PDFTranscriptional repressors play an important role in regulating phage life cycle. Here, we examine how synthetic transcription repressors can be used in bacteriophage T7 to create a dynamic, controllable infectivity switch. We engineered T7 phage by replacing a large region of the early phage genome with different combinations of ligand-responsive promoters and ribosome binding sites (RBS) designed to control the phage RNA polymerase, .
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