Several Characteristics of G.L. Barron Important for Heather Plants' Controlled Mycorrhization.

G.L. Barron is a recognized fungal species capable of forming ericoid mycorrhiza with various positive effects on host plants; therefore, newly found and previously uncharacterized strains may be valuable for heather plants' controlled mycorrhization.

[Morpho-functional changes of Staphylococcus aureus biofilms under the effect of batumin].

Aim: Study the effect of batumin isolated from metabolites of Pseudomonas batumici bacteria on the formation of biofilms by staphylococci under the control of atomic force microscopy. MATERIALS AND METHODS; S. aureus 25923 (ATCC) and S.

[Functional characterization of Pseudomonas batumici 17/20 upon long-term storage].

Variability of properties and antibiotic activity, as well as cells survival of Pseudomonas batumici 17/20--the producer of batumin (antistaphylococcal antibiotic) after long-term storage under vaseline oil layer have been studied. The main culture-morphological and physiological biochemical properties of the mutant strain have been investigated. It has been shown that storage under vaseline oil allows to preserve high level of antibiotic activity: batumin synthesis by the producer was 150 mg/l.

[Algorithm of identification of atypical variants of Staphylococci, pathogens of chronic pyo-inflammatory processes in humans].

Staphylococcal pathogens of chronic relapsing infections, such as cystic pneumosclerosis and osteomyelitis are characterized by atypical morphology of the colonies (atypical variants of staphylococci) and present a subpopulation in clinically significant staphylococci. Since the loss of some phenotypic characteristics important for the genus Staphylococcus due to mutations, identification of such staphylococcal variants is difficult and sometimes impossible. An algorithm of identification of atypical variants of S.

Application of the antibiotic batumin for accurate and rapid identification of staphylococcal small colony variants.

Background: Staphylococcus aureus is a major human pathogen causing significant morbidity and mortality. The S. aureus colonies in osteomyelitis, in patients with cystic fibrosis and patients with endoprosthesis rejection frequently have an atypical morphology, i.

[Influence of antistaphylococcus antiobiotic batumin on microorganism biofilm formation].

Aim: Study the influence of batumin on microorganism biofilm formation.

Materials And Methods: Experimental data on the antimicrobial effect of batumin on microorganisms and biofilm formation (BFF) was obtained by studying 80 strains of bacteria and fungi isolated from microbial biocenosis of the nose of staphylococcus carriers and patients during examination for intestine dysbiosis. 80% pure batumin was used in the experiments.

Pseudomonas batumici sp. nov., the antibiotic-producing bacteria isolated from soil of the Caucasus Black Sea coast.

Four novel strains of saprophytic bacteria were isolated from the soil samples collected in the moist subtropics region (the Black Sea coast of the Caucasus) and studied using methods of polyphasic taxonomic analysis. Microorganisms were Gram-negative, oxidase positive, aerobic, rod-shaped motile bacteria that produced antibiotic named batumin with high and selective activity against staphylococci; its total formula was C30H48N2O7 Phylogenetic analysis of 16S rRNA gene sequences (1376 bp, accession number in Genbank--JF306642) indicated that the isolates belonged to the gamma-Proteobacteria formed a separate branch within the genus Pseudomonas and had 98% 16S rRNA gene sequence similarity with Pseudomonas gingeri. The latter essentially differed from the studied strains in its phenotypic characteristics.

[Characteristics of atypical forms of staphylococci (SCVs) isolated from patients with osteomyelitis].

Small-colony variants (SCVs), isolated from a population of the parental strains of Staphylococcus aureus, S. haemolyticus and S. epidermidis lost a number of features typical of the species and genus and were characterized by delayed growth, altered colony morphology, lack of pigmentation and changed carbohydrate consumption.

[Characterization of batumin resistant variants of Staphylococcus aureus clinical isolates].

Population analysis of 20 batumin susceptible staphylococcal strains, isolated from clinical materials allowed to detect resistant clones only in 3 strains. The frequency of batumin resistant variants isolation was low and depended on the antibiotic concentration. Resistance to batumin in 5 clinical strains and in 1 reference strain of Staphylococcus aureus ATCC 6538 P (209P) developed under laboratory conditions and induced significant phenotypic and chemotaxonomic changes in the pathogen.

Antibiotic batumin for diagnostics of staphylococci and treatment of Staphylococcus aureus nasal carriage.

Paper disks containing 5 microg of antibiotic batumin (preparation "Diastaph") were tested using standard disk diffusion technique against 1520 strains of bacteria (clinical isolates of different genera and type strains of staphylococci and micrococci). Presence of growth inhibition zone 17 mm and more around the disk gave evidence that the isolate belonged to the genus Staphylococcus. Method ensured quick and reliable identification of all strains of this genus.

[Antimicrobial spectrum of antibiotic batumin].

Antibacterial activity of antibiotic batumin, isolated from Pseudomonas genus has been studied using CLSI standard methods. Batumin was highly active against all studied strains belonging to 10 species of Staphylococcus genus (minimal inhibitory concentration (MIC)--0.25-0.

Phenotypic peculiarities of batumin-resistant staphylococci.

Batumin-resistant variants of two clinical strains of Staphylococcus (S.) aureus and of strain ATCC 6538 P (209 P) were produced in vitro by continuous subculture in the presence of batumin. Their phenotypic characteristics differed substantially from the parent strains properties and did not correspond to those of S.

[Study on activity of antistaphylococcal antibiotic batumin against methicillin resistant strains of staphylococci].

A stable increase in the frequency of methicillin resistant clinical isolates of staphylococci was recorded. 258 strains of Staphylococcus aureus and S. epidermidis, including methicillin resistant ones, were susceptible to batumin.

Double-stranded RNA viruses in a mycocinogenic strain of Cystofilobasidium infirmominiatum.

The viral particles (about 30 nm in diameter) that contain dsRNAs (2.0 and 6.3 kbp) encapsidated by a coat of protein were detected in a mycocin-secreting strain of Cystofilobasidium infirmominiatum isolated from plants in an oak forest (Moscow region).

[Efficacy of diagnostic plates with batumin in identification and indication of staphylococci].

In investigations designed to study sensitivity of 763 clinical strains of microorganisms that belonged to different taxonomic groups with respect to batumin, a new antibiotic recovered from bacteria of the genus Pseudomonas, all members of the genus Staphylococcus displayed high sensitivity to batumin irrespective of their sensitivity or resistance to other antibiotics. High selectivity of batumin in respect to representatives of the genus Staphylococcus has been used to create a novel diagnostic preparation diastaph--a batch of paper disks with batumin (5 mkg) spread on them. Results of the trial given to the preparation diastaph suggest to us a high specificity of this identification option for staphylococci of different species in mono- and mixed cultures, a possibility of our using them simultaneously with or instead of the Hew-Leuffson medium, and an expediency of employment of batumin disks for a prompt preindication of staphylococci in a clinical setting while studying a material notable for a high probability that there are bacterial associations involving staphylococci.

[Specificity of sensitivity to mycocin from Tilletiopsis flava BKM Y-2838].

The mycocinogenous strain Tilletiopsis flava VKM Y-2823 was found to possess fungicidal activity at pH 3.5-4.5, which was retained after curing the strain by eliminating the extrachromosomal genetic elements.

[Ecological diagnosis of pyoseptic diseases in newborns using the "Diastaph" disc].

The etiological spectrum of pyoseptic diseases was studied in 113 newborns. It was established, that between 187 strains of microorganisms, belonging to 19 species, 73.3% were staphylococci and streptococci, 25.

[Transposon mutagenesis in preparing mutants of a strain--a producer of a new antibiotic batumin].

Various plasmids carrying transposon Tn5 were used to generate insertion mutants synthesizing batumin, a unique antibiotic with a selective antistaphylococcal effect. One of the plasmids used provided a sufficient yield of the clones in question. An analysis of over 7000 clones allowed us to select the mutant clones with increased and decreased levels of batumin synthesis and the mutants that lost the ability to synthesize this antibiotic.

[The use of batumin-containing disks for the rapid identification of staphylococci].

The study of 467 microbial strains obtained from collections and from clinical sources revealed that microorganisms of the genus Staphylococcus were highly sensitive to batumin, a new antibiotic obtained from bacteria of the genus Pseudomonas. 378 strains of 15 Staphylococcus species proved to be highly sensitive to the diagnostic preparation "Diastaph", developed on the basis of batumin (antibiotic-impregnated discs); After 18-hour incubation the diameter of the growth inhibition zones on agar-containing culture media was 18-38 mm. Strains belonging to the genera Micrococcus, Dermacoccus, Kocuria and Kytococcus, as well as the tested representatives of other taxa (Planococcus, Streptococcus, Corynebacterium, Acinetobacter, Pseudomonas, Neisseria, the representatives of all tested genera of the family Enterobacteriaceae, fungi of the genus Candida) were insensitive to the diagnosticum.

[Isolation of highly active strain producing the antistaphylococcal antibiotic batumin].

The use of chemical and UV-induced mutageneses allowed us to increase the biosynthetic activity of the strain capable of producing new antistaphylococcal antibiotic, batumin. The strain of Pseudomonas batumici N17 producing 87-100 mg batumin per liter culture liquid was selected. Its activity was 3.

[Biosynthesis of the new antistaphylococcal antibiotic batumin].

Levels of batumin biosynthesis by separate cells of the antibiotic-producing strain were investigated. The strain belongs to the genus Pseudomonas. It was found that the cell population was not homogeneous by that property.

[The effect of the antibiotic AL-87 on the amino acid yield from Staphylococcus aureus 209P cells].

Antibiotic AL-87 has been studied for its effect on the composition of intracellular free amino acids and of amino acids in culture fluid of Staphylococcus aureus 209 P. It is established that the content of amino acids in the culture fluid of S. aureus 209 P is doubled due to antibiotics, while the content of intracellular free amino acids considerably decreases.

[Antibiotic AL-87 induction of changes in the fatty acid composition of phospholipids and neutral lipids in a sensitive strain of Staphylococcus aureus 209P].

Under the action of the sub-bacteriostatic concentration (0.02 microgram/ml) of antibiotic AL-87 there formed in all the fractions of phospholipids and neutral lipids of S. aureus 209P unsaturated branched fatty acids not detected in the control and the content of shorter chain saturated branched fatty acids increased.

[Importance of Bdellovibrio in regulating microbial cenoses and self-purification processes in domestic sewage].

The bacterial parasite Bdellovibrio was directly proved to be involved in the regulation of microbial cenoses and in the self-purification of domestic waste waters. The incidence of heterotrophs, Gram-negative bacteria, E. coli and Bdellovibrio was followed up in dynamics in the microecological system of waste waters for ten days.