[Nucleotide composition and contents of CpG and CpNpG in the ITS1, ITS2 and 5.8S rRNA of the representatives of phylogenetic branches of melanthiales-liliales and melanthiales and melanthiales-asparagales (angiospermae, monocotyledones) reflects a characteristic feature of evolution].

Variations of nucleotide composition and frequency of CpG and CpNpG sequences in the clusters of nuclear ribosomal genes of taxa, belonging to two phylogenetic branches of Angiospermae have been statistically analyzed. This region of eucaryotic genomes is nucleolus organizer and functions in a separate compartment of cell nucleus that can do running here processes it is enough specific. It is shown that level of evolution advance of a taxon, defined on morphological data, is in positive correlation with quantitative value of dC, CpG and CpNpG.

[The pattern of the lateral phylogenetic branch development].

The lateral phylogenetic branch is S-formed, when regarded in the time-divergency degree coordinates. On the initial side of the curve, the rate of divergency development is low to increase in the middle, and to fall down again on the final side. In result, within one phylogenetic branch all the taxa appear to be typologically diverse.

[Potential functional differentiation of genome in the course of evolution and approaches to its study. II. Elucidation of diversity of phylogenetic lines].

It has been shown elsewhere (Chupov, 2001) that the branching of phylogenetical trunks goes by anisotomical way. Thus, in one of newly formed branches a possibility remains of a further evolutionary transformation, while taxa belonging to another branch sink into a prolonged evolutionary stasis. In the author's opinion, such a phenomenon is to be accompanied by distinctions in constitution of genetical cell devices of the taxa belonging to the branches with evolutionary contrasting potencies.

[Potential functional differentiation of genome in the course of evolution and approaches to its study. I. Neontological annals of evolution and its analysis].

It is shown that the division of phylogenetical branches descends anisotomically. One new branch becomes evolutionary not active. Another one continues actively to develop.

Chemical modification of polymers with physiologically active species using water-soluble carbodiimides.

The covalent immobilization of some proteins (ovomucoid from duck egg white, human serum albumin, aldolase and thiroglobulin) on the surface of polyethylene grafted with polyacrylic acid has been studied. Water-soluble carbodiimide was (1-ethyl-3-(3-dimethylaminopropyl carbodiimide) used as a condensing agent. It was shown that three different reactions can occur in the reaction mixture during immobilization: the reaction between carboxy groups of graft copolymer and amino groups of protein (the immobilization reaction itself) and reactions between carboxy and amino groups of protein molecules (reactions of intra- and intermolecular cross-linking).

[Interaction between globular proteins and water-soluble carbodiimides].

Interactions of a number of globular proteins with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide were studied. Under conditions of carbodiimide excess at a protein concentration lower than 88.5 x 10(-5) mol/l, the crosslinking reaction was found to proceed exclusively by an intramolecular mechanism.

[Preparation and characteristics of immobilized collagenase isolated from crab Paralithodes camtschatica].

Collagenolytic protease from hepatopancreas of king crab was immobilized on water-soluble reactive polymers containing N-succinimide units and possessing different hydrophobicity-hydrophilicity balance. The immobilized enzyme displayed higher heat stability, but its proteolytic activity decreased with the increase in the number of the copolymer hydrophobic groups. Highly active thermostable preparations of collagenolytic protease were obtained by copolymerization.

Biospecific haemosorbents based on proteinase inhibitor. I. Synthesis and properties.

Sorbents for the removal of proteolytic enzymes from biological fluids were synthesized by immobilization of proteinase inhibitor, ovomucoid from duck egg white, in polymeric hydrogel matrix. The immobilization of the protein inhibitor modified by acryloyl chloride occurs during copolymerization of the unsaturated derivative of inhibitor with hydrophilic monomer and crosslinking agent. It was shown that the sorbent obtained possesses a high affinity for proteinases and is haemocompatible.

Covalent immobilization of microorganisms in polymeric hydrogels.

A method of covalent immobilization of microorganisms (marine luminescent bacteria and yeast) in polymeric hydrogels is described. It is shown that cell immobilization leads to the creation of materials having properties of both synthetic polymers and physiologically active systems. Application of systems containing covalent immobilized yeast and photobacteria in biotechnological and other processes is proposed.

[Isolation and purification of phospholipase C from Clostridium perfringens and antibodies against it using polymeric biospecific adsorbents].

A new polymeric biospecific adsorbent intended for isolation of Clostridium perfringens phospholipase C (PLC) and PLC-specific antibodies is discussed. It was obtained by radical copolymerization of acrylamide, methylenbisacrylamide, and the acryl acid chloranhydride-acylated substrate of PLC (chicken yolk lecithovitellin). Maximal adsorption of PLC was observed in the presence of the enzyme activator, and the highest amount of PLC was eluted in case of its minimal adsorption.