[Applicability of thermal dissipation probe in the determination of trunk flow of under cyclic heating mode].

To evaluate the adaptability of the cyclic heating mode in the thermal diffusion probe method (TDP) in the measurement of trunk sap flow and the accuracy of the measurement of tree transpiration water consumption, we selected as the research object and set three different heating modes: 60 min/0 min (continuous heating mode), 30 min/30 min (cyclic heating mode with 30 min heating and 30 min cooling), 10 min/50 min (cyclic heating mode with 10 min heating and 50 min cooling). Based on the measured value of the whole tree container wei-ghing method, the temperature gradient characteristics of different heating modes were analyzed using the measurement technology of thermal diffusive trunk sap flow. The Granier's corrected formulas of cyclic heating modes were constructed, with its error being analyzed by validity verification.

Ubiquitin-conjugating enzyme E2T regulates cell proliferation and migration in cholangiocarcinoma.

Ubiquitin-conjugating enzyme E2T (UBE2T) is overexpressed in several human cancer cells, but a role in cholangiocarcinoma (CAA) progression has not been investigated. We analyzed the expression of UBE2T in CAA tissues. Then, we generated UBE2T deregulation models in which it was overexpressed or silenced, and examined the effects on CAA malignant progression by flow cytometry, western blot, MTT assay, wound healing assay and transwell assay.

Role of ubiquitin-conjugating enzyme E2T in the carcinogenesis and progression of pancreatic cancer.

Ubiquitin-conjugating enzyme E2T (UBE2T) is a recently discovered oncogenic protein. Numerous studies reported that UBE2T is highly expressed in various types of human cancer; however, its role in the carcinogenesis and progression of pancreatic cancer remains unknown. The aim of the present study was to investigate the role of UBE2T in pancreatic cancer progression through experiments in pancreatic cancer tissues and cell lines.

[Differences in atmospheric CO concentration and δC value between green spaces and its adjacent roads as well as the influencing factors in Beijing, China].

Near-surface atmospheric CO concentration and δC value in four greenspaces and on their adjacent roads in Beijing were measured by off-axis integrated cavity output spectroscopy to analyze the influence of urban greenspace on spatial distribution of near-surface atmospheric CO. The results showed that atmospheric CO concentration and δC value varied substantially both temporally and spatially. The highest CO concentration was found in the urban area, followed by the suburbs, and the lowest CO concentration was in the outer suburbs.

[Closed reduction combined with Taylor three-dimensional space stent fixation for supracondylar femoral fracture in children].

Objective: To explore the effectiveness and safety of closed reduction combined with Taylor three-dimensional space stent fixation in treatment of supracondylar femoral fractures in children.

Methods: Between July 2008 and July 2016, 20 patients with supracondylar femoral fractures were treated with closed reduction combined with Taylor three-dimensional space stent fixation. There were 14 males and 6 females, with an average age of 10.

Hepatocyte isolation from resected benign tissues: Results of a 5-year experience.

Aim: To analyze retrospectively a 5-year experience of human hepatocyte isolation from resected liver tissues with benign disease.

Methods: We established a method of modified four-step retrograde perfusion to isolate primary human hepatocytes. Samples were collected from the resected livers of patients with intrahepatic duct calculi (n = 7) and liver hemangioma (n = 17).

A novel AKT inhibitor, AZD5363, inhibits phosphorylation of AKT downstream molecules, and activates phosphorylation of mTOR and SMG-1 dependent on the liver cancer cell type.

Due to frequent phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) signaling pathway dysregulation, AKT is typically accepted as a promising anticancer therapeutic target. mTOR, in particular, represents a suitable therapeutic target for hepatocellular carcinoma, whilst suppressor with morphogenetic effect on genitalia family member-1 (SMG-1) is believed to serve a potential tumor suppressor role in human cancer. Despite SMG-1 and mTOR belonging to the same PI3K-related kinase family, the interactions between them are not yet fully understood.

[Vacuum sealing drainage combined with free skin graft in repairing cutaneous deficiency of traumatic shank amputation stump].

Objective: To observe clinical efficacy in treating cutaneous deficiency of traumatic shank amputation stump with full-thickness skin graft combined with vacuum sealing drainage.

Methods: From September 2009 to December 2012, 15 patients with cutaneous deficiency of traumatic shank amputation stump were treated with full-thickness skin graft combined with vacuum sealing drainage. Among patients, there were 11 males and 4 females with an average age of 41.

Thyroid-stimulating hormone regulates hepatic bile acid homeostasis via SREBP-2/HNF-4α/CYP7A1 axis.

Background & Aims: Bile acids (BAs) play a crucial role in dietary fat digestion and in the regulation of lipid, glucose, and energy metabolism. Thyroid-stimulating hormone (TSH) is a hormone produced by the anterior pituitary gland that directly regulates several metabolic pathways. However, the impact of TSH on BA homeostasis remains largely unknown.

Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination.

Aim: To establish a method for the reversible immortalization of human hepatocytes, which may offer a good and safe source of hepatocytes for practical applications.

Methods: We successfully isolated primary human hepatocytes from surgically resected liver tissue taken from a patient with liver hemangiomas. The freshly isolated cells were then immortalized with retroviral vector SSR#69 expressing simian virus 40 large T antigen (SV40T) and hygromycin-resistance genes flanked by paired loxP recombination targets.

Free and glycosidically bound aroma compounds in cherry (Prunus avium L.).

This paper reports the occurrence of both free and glycosidically bound aroma compounds in three sweet cherry cultivars ('Hongdeng', 'Hongyan' and 'Rainier'), with 97 compounds being identified in the three cultivars. The major free volatile compounds found were hexanal, (E)-2-hexenal, (E)-2-hexen-1-ol, benzyl alcohol and benzaldehyde. The major bound volatile compounds found were benzyl alcohol, geraniol, 2-phenylethanol.

[Variations of canopy temperature in Quercus variabilis plantation and their relations with micrometeorological factors].

Based on the canopy temperature and micrometeorological data of Quercus variabilis in its main growth season (from May to August) in hilly areas of North China in 2011, this paper analyzed the variations of canopy temperature (T(c)) in Q. variabilis plantation and their relations with micrometeorological factors in typically clear days and cloudy days. From 9:00 to 17:00 in clear days, the boundary layer of canopy was unstable, and the mean T(c) was 3.

[Thumb replantation through bridging ulnar proper digital artery and dorsal carpal branch of radial artery].

Objective: To introduce treatment method of replantation of severed thumb trauma with proximal arterial, and to evaluate its efficacy.

Methods: From February 2007 to March 2009,13 patients with severed thumb in serious injury of proximal arterial were treated with vein graft of volar forearm,bridging between dorsal carpal branch of radial artery on nasopharyngeal fossa and distal stump of ulnar proper digital artery. Among them, there were 11 males and 2 females with an average age of 34.

Extended local resection for treatment of periampullary carcinoma of vater.

Background: The trauma caused by pancreatoduodenectomy for periampullary carcinoma of vater is often severe and extensive. The purpose of this study was to evaluate the effect of extended local resection in the treatment of periampullary carcinoma of vater.

Methods: The extra-hepaticobiliary tract, the confluence of the pancreatic and biliary duct, vater ampulla and duodenal papilla were resected en bloc in 8 patients with periampullary carcinoma from 1995 to 1998.

Surgical treatment for uncinate process carcinoma of the pancreas.

Objectives: To analyze the clinical features of uncinate process carcinoma of the pancreas and to improve the resection rate.

Methods: From January 1990 to June 1999, 10 patients with pancreas uncinate process carcinoma received Whipple's operation. Portal vein (PV) resection and reanastomosis were performed in 5 patients, and the resected length varied from 2.