5-AZA-2'-deoxycytidine (5-AZA-CdR) leads to down-regulation of Dnmt1o and gene expression in preimplantation mouse embryos.

5-AZA-2'-deoxycytidine (5-AZA-CdR) is a demethylating, teratogenic agent and a mutagen, which causes defects in the developing mouse and rat after implantation. Our previous data indicated that 5-AZA-CdR (0.2 and 1.

Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition.

Intramuscular fat (IMF) content plays a key role in establishing pork quality. In the present study, differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to identify differentially expressed (DE) genes between longissimus dorsi (LD) muscles with extremely different IMF content. A major DE gene associated with IMF content was identified as splicing factor serine-arginine rich protein (SFRS18) gene, also known as SRrp130.

[Effects of 22 microsatellite DNA markers on stress traits in F2 population of pigs].

In the present study, we analyzed the effects of 22 microsatellite DNA loci on behavior responses and plasma concentration of hormones related to stress and metabolism following transport trial in pigs. These subjects were selected from the segregating F2 generation of a Pietrain (PIE) x Erhualian (EHL) cross, and the two swine strains differ in stress tolerance. A so-called Backtest Score (BS) at 3, 10 and 17 days after birth (BS3, BS10 and BS17) was implemented to assess the behavior responses for each piglet.

The pig p160 co-activator family: full length cDNA cloning, expression and effects on intramuscular fat content in Longissimus Dorsi muscle.

Evidence has shown in mouse that the p160 co-activator family, consisting of nuclear receptor co-activators 1 (NCOA1), 2 (NCOA2) and 3 (NCOA3) plays a critical role in adipogenesis. In the present study, we cloned these three porcine genes, identified their transcript variants and analyzed their expression level in relation to intramuscular fat (IMF) content in Longissimus Dorsi (LD) muscle. Both in silico cloning and PCR amplification revealed a full length cDNA sequence of 6591bp for NCOA1 (EU346671), 7628bp for NCOA2 (EU346672) and 5005bp for NCOA3 (EU346674) in pigs, respectively.