PUS7-dependent pseudouridylation of ALKBH3 mRNA inhibits gastric cancer progression.

Background: RNA pseudouridylation is a critical post-transcriptional modification that influences gene expression and impacts various biological functions. Despite its significance, the role of mRNA pseudouridylation in cancer remains poorly understood. This study investigates the impact of pseudouridine synthase 7 (PUS7)-mediated pseudouridylation of Alpha-ketoglutarate-dependent Dioxygenase alkB Homolog 3 (ALKBH3) mRNA in gastric cancer.

m6Am methyltransferase PCIF1 negatively regulates ciliation by inhibiting BICD2 expression.

N6, 2'-O-dimethyladenosine (m6Am) is a widespread RNA modification catalyzed by the methyltransferase PCIF1 (phosphorylated CTD interacting factor 1). Despite its prevalence, the biological functions of m6Am in RNA remain largely elusive. Here, we report a critical role of PCIF1-dependent m6Am RNA modification in ciliogenesis in RPE-1 cells.

Progress on RNA-based therapeutics for genetic diseases.

RNA therapeutics inhibit the expression of specific proteins/RNAs by targeting complementary sequences of corresponding genes or encode proteins for the synthesis desired genes to treat genetic diseases. RNA-based therapeutics are categorized as oligonucleotide drugs (antisense oligonucleotides, small interfering RNA, RNA aptamers), and mRNA drugs. The antisense oligonucleotides and small interfering RNA for treatment of genetic diseases have been approved by the FDA in the United States, while RNA aptamers and mRNA drugs are still in clinical trials.

mA RNA Modification in Gene Expression Regulation.

-methyladenosine (mA) is a prevalent and reversible post-transcriptional RNA modification that decorates tRNA, rRNA and mRNA. Recent studies based on technical advances in analytical chemistry and high-throughput sequencing methods have revealed the crucial roles of mA RNA modification in gene regulation and biological processes. In this review, we focus on progress in the study of mA methyltransferases, mA demethylases and mA-dependent RNA-binding proteins and highlight the biological mechanisms and functions of mA RNA modification, as well as its association with human disease.

ALKBH3-dependent mA demethylation of Aurora A mRNA inhibits ciliogenesis.

Primary cilia are antenna-like subcellular structures to act as signaling platforms to regulate many cellular processes and embryonic development. mA RNA modification plays key roles in RNA metabolism and gene expression; however, the physiological function of mA modification remains largely unknown. Here we find that the mA demethylase ALKBH3 significantly inhibits ciliogenesis in mammalian cells by its demethylation activity.