Generation of an induced pluripotent stem cell line (OGHFUi001-A) from a type 1 early infantile epileptic encephalopathy with ARX mutation.

Type 1 early infantile epileptic encephalopathy (EIEE1) is a severe early-onset epileptic encephalopathy with arrest of psychomotor development caused by hemizygous mutations in the ARX gene, which encodes a transcription factor in fundamental brain developmental processes. A human induced pluripotent stem cell (iPSC) line, termed as OGHFUi001-A, was generated using non-integrating episomal vector technique from peripheral blood mononuclear cells (PBMCs) of a 7-year-old male EIEE1 patient, who had a hemizygous (c.989G > T: p.

Geometry of the Contact Zone between Fused Membrane-Coated Beads Mimicking Cell-Cell Fusion.

The fusion of lipid membranes is a key process in biology. It enables cells and organelles to exchange molecules with their surroundings, which otherwise could not cross the membrane barrier. To study such complex processes we use simplified artificial model systems, i.

Optical fusion assay based on membrane-coated spheres in a 2D assembly.

A major goal in neurophysiology and research on enveloped viruses is to understand and control the biology and physics of membrane fusion and its inhibition as a function of lipid and protein composition. This poses an experimental challenge in the realization of fast and reliable assays that allow us, with a minimal use of fluorescent or radioactive labels, to identify the different stages of membrane-membrane interaction ranging from docking to complete membrane merging. Here, an optical two-dimensional fusion assay based on monodisperse membrane-coated microspheres is introduced, allowing unequivocal assignment of docking and membrane fusion.

Alkyl chain length dependent morphology and emission properties of the organic micromaterials based on fluorinated quinacridone derivatives.

The fluorinated quinacridone derivatives N,N'-dialkyl-2,9-difluoroquinacridone (Cn-DFQA, n = 4, 8, 10, 16) with different alkyl chains were used as building blocks to assemble luminescent micromaterials. It was demonstrated that the morphology and emission of the Cn-DFQA-based micromaterials strongly depended on their alkyl chain length. C4-DFQA and C8-DFQA showed stronger tendency to form 1-D microstructures, while C10-DFQA and C16-DFQA displayed the aggregation properties to form diamond and hexagonal platelike microcrystals, respectively.

Three-dimensional colloidal crystal-assisted lithography for two-dimensional patterned arrays.

In this paper, we report our recent work on preparing two-dimensional patterned microstructure arrays using three-dimensional colloidal crystals as templates, namely, colloidal crystal-assisted lithography. Two alternative processes are described and involved in colloidal crystal-assisted lithography. One is based upon imprinting the polymer films with three-dimensional silica colloidal crystals, and the other is based upon chemically depositing Ag microstructures on Au substrates covered by polymer colloidal crystals.