Publications by authors named "Chunxiang You"

The dehydration responsive element binding (DREB) transcription factors play an important role in plant growth and development and are extensively involved in plant responses to abiotic stress. The DREB family contains six subfamilies, and TINY belongs to the DREB-A4 subfamily. The gene, , plays a role in regulating plant growth and responses to stress.

View Article and Find Full Text PDF

, a valuable germplasm resource in the genus , is indigenous to China and widely distributed. However, little is known about the lineage composition and genetic basis of 'ZA', a mutant type of . In this study, we compared the differences between 'ZA' and wild type from the perspective of morphology and ultrastructure and analyzed their chloroplast pigment content based on biochemical methods.

View Article and Find Full Text PDF

Fruit colour is a critical determinant for the appearance quality and commercial value of apple fruits. Viroid-induced dapple symptom severely affects the fruit coloration, however, the underlying mechanism remains unknown. In this study, we identified an apple dimple fruit viroid (ADFVd)-derived small interfering RNA, named vsiR693, which targeted the mRNA coding for a bHLH transcription factor MdPIF1 (PHYTOCHROME-INTERACTING FACTOR 1) to regulate anthocyanin biosynthesis in apple.

View Article and Find Full Text PDF
Article Synopsis
  • Fungal pathogens induce necrosis in apple fruits, creating a red circle that indicates a response to infection, though the molecular process behind this is not well understood.
  • This study identified that salicylic acid (SA) accumulation from fungal infection stimulates anthocyanin production via the MdNPR1-MdTGA2.2 pathway in apples, correlating with the red circle's coloration.
  • Suppressing or overexpressing either MdNPR1 or MdTGA2.2 affected the fruit's color, confirming their critical roles in regulating anthocyanin biosynthesis in response to pathogens.
View Article and Find Full Text PDF

Although the strigolactone (SL) signaling pathway and SL-mediated anthocyanin biosynthesis have been reported, the molecular association between SL signaling and anthocyanin biosynthesis remains unclear. In this study, we identified the SL signal transduction pathway associated with anthocyanin biosynthesis and the crosstalk between gibberellin (GA) and SL signaling in apple (Malus × domestica). ELONGATED HYPOCOTYL5 (HY5) acts as a key node integrating SL signaling and anthocyanin biosynthesis, and the SL-response factor AGAMOUS-LIKE MADS-BOX9 (AGL9) promotes anthocyanin biosynthesis by activating HY5 transcription.

View Article and Find Full Text PDF

The E3 ubiquitin ligase MdSINA11 targets the jasmonate ZIM domain protein MdJAZ2 for ubiquitination and degradation through the 26S proteasome pathway, thereby initiating jasmonate signaling and jasmonic acid-triggered anthocyanin biosynthesis in apple.

View Article and Find Full Text PDF

Jasmonic acid (JA) and gibberellin (GA) coordinately regulate plant developmental programs and environmental cue responses. However, the fine regulatory network of the cross-interaction between JA and GA remains largely elusive. In this study, we demonstrate that MdNAC72 together with MdABI5 positively regulates anthocyanin biosynthesis through an exquisite MdNAC72-MdABI5-MdbHLH3 transcriptional cascade in apple.

View Article and Find Full Text PDF

Salt stress is a critical limiting factor for fruit yield and quality of apples. Brassinosteroids (BRs) play an important role in response to abiotic stresses. In the present study, application of 2,4- Epicastasterone on seedlings of Malus 'M9T337' and Malus domestica 'Gala3' alleviated the physiological effects, such as growth inhibition and leaf yellowing, induced by salt stress.

View Article and Find Full Text PDF
Article Synopsis
  • BTB and TAZ domain proteins (BTs) are crucial adaptors for the CUL3-RING ubiquitin ligase complex, which helps target proteins for ubiquitination under various conditions.
  • The study reveals that the calmodulin-like protein MdCML15 negatively regulates the function of the apple scaffold protein MdBT2, influencing processes related to membrane H-ATPase regulation and tolerance to iron deficiency.
  • The findings suggest that MdCML15, through its interactions with MdBT2 and the target protein MdbHLH104, plays a significant role in lowering rhizosphere pH and maintaining iron balance in apple plantlets.
View Article and Find Full Text PDF

The Tetratricopeptide repeat (TPR)-like superfamily with TPR conserved domains is widely involved in the growth and abiotic stress in many plants. In this report, the gene MdTPR16 belongs to the TPR family in apple (Malus domestica). Promoter analysis reveal that MdTPR16 incorporated various stress response elements, including the drought stress response elements.

View Article and Find Full Text PDF

Heat shock protein 20 (Hsp20) is a small molecule heat shock protein that plays an important role in plant growth, development, and stress resistance. Little is known about the function of Hsp20 family genes in apple (Malus domestica). Here, we performed a genome-wide analysis of the apple Hsp20 gene family, and a total of 49 Hsp20s genes were identified from the apple genome.

View Article and Find Full Text PDF
Article Synopsis
  • Overexpression of the MdLBD3 gene in Arabidopsis enhances the plant's resistance to salt and drought stress while also promoting early flowering.
  • The study identifies and analyzes the LATERAL ORGAN BOUNDARY DOMAIN (LBD) protein family in apples, noting the limited understanding of their roles in woody plants.
  • Findings suggest that MdLBD3, localized in the nucleus and expressed in various tissues, could be a valuable genetic resource for breeding apples with improved tolerance to environmental stresses.
View Article and Find Full Text PDF

ABSCISIC ACID-INSENSITIVE5 (ABI5) is a core regulatory factor that mediates the ABA signaling response and leaf senescence. However, the molecular mechanism underlying the synergistic regulation of leaf senescence by ABI5 with interacting partners and the homeostasis of ABI5 in the ABA signaling response remain to be further investigated. In this study, we found that the accelerated effect of MdABI5 on leaf senescence is partly dependent on MdbHLH93, an activator of leaf senescence in apple.

View Article and Find Full Text PDF

Artificially enhancing photosynthesis is critical for improving crop yields and fruit qualities. Nanomaterials have demonstrated great potential to enhance photosynthetic efficiency; however, the mechanisms underlying their effects are poorly understood. This study revealed that the electron transfer pathway participated in nitrogen-doped carbon dots (N-CDs)-induced photosynthetic efficiency enhancement (24.

View Article and Find Full Text PDF

Plants possess the remarkable ability to sense detrimental environmental stimuli and launch sophisticated signal cascades that culminate in tailored responses to facilitate their survival, and transcription factors (TFs) are closely involved in these processes. Phytochrome interacting factors (PIFs) are among these TFs and belong to the basic helix-loop-helix family. PIFs are initially identified and have now been well established as core regulators of phytochrome-associated pathways in response to the light signal in plants.

View Article and Find Full Text PDF

Abscisic acid (ABA), as a plant hormone, plays a positive role in leaf chlorosis; however, the underlying molecular mechanism is less known. Our findings provide ABA treatment reduced the chlorophyll accumulation in apple, and Sucrose Non-fermenting 1-Related Protein Kinase 1.1 ( participates in the process.

View Article and Find Full Text PDF

Anthocyanins are secondary metabolites induced by environmental stimuli and developmental signals. The positive regulators of anthocyanin biosynthesis have been reported, whereas the anthocyanin repressors have been neglected. Although the signal transduction pathways of gibberellin (GA) and jasmonic acid (JA) and their regulation of anthocyanin biosynthesis have been investigated, the cross-talk between GA and JA and the antagonistic mechanism of regulating anthocyanin biosynthesis remain to be investigated.

View Article and Find Full Text PDF

Drought stress is one of the main environmental factors limiting plant growth and development. Plants adapt to changing soil moisture by modifying root architecture, inducing stomatal closure, and inhibiting shoot growth. The AP2/ERF transcription factor DREB2A plays a key role in maintaining plant growth in response to drought stress, but the molecular mechanism underlying this process remains to be elucidated.

View Article and Find Full Text PDF

Cuticular waxes play important roles in plant development and the interaction between plants and their environment. Researches on wax biosynthetic pathways have been reported in several plant species. Also, wax formation is closely related to environmental condition.

View Article and Find Full Text PDF

Plants have evolved a complex and elaborate signaling network to respond appropriately to the pathogen invasion by regulating expression of defensive genes through certain transcription factors. The APETALA2/ethylene response factor (AP2/ERF) family members have been determined as key regulators in growth, development, and stress responses in plants. Moreover, a growing body of evidence has demonstrated the critical roles of AP2/ERFs in plant disease resistance.

View Article and Find Full Text PDF

Various abiotic stresses, especially heavy metals near factories around the world, limit plant growth and productivity worldwide. Zinc is a light gray transition metal, and excessive zinc will inactivate enzymes in the soil, weaken the biological function of microorganisms, and enter the food chain through enrichment, thus affecting human health. Lipoxygenase (LOX) can catalyze the production of fatty acid derivatives from phenolic triglycerides in plants and is an important pathway of fatty acid oxidation in plants, which usually begins under unfavorable conditions, especially under biotic and abiotic stresses.

View Article and Find Full Text PDF
Article Synopsis
  • Auxin/indole-3-acetic acid (AUX/IAA) and auxin response factor (ARF) proteins play vital roles in the auxin signaling pathway, but their specific modifications and effects on anthocyanin biosynthesis are not fully understood.
  • The study identifies MdARF5-1 as a negative regulator of anthocyanin production in apples, mediating signals from auxin and ethylene by inhibiting the expression of MdERF3.
  • Additionally, E3 ubiquitin ligases MdSINA4 and MdSINA11 differentially regulate anthocyanin biosynthesis through the ubiquitination of MdIAA29 and MdARF5-1, illustrating a complex interaction
View Article and Find Full Text PDF

In this study, we analyzed the effects of treatments with titanium dioxide nanoparticles (NPs-TiO) and ethylene on anthocyanin biosynthesis and reactive oxygen species (ROS) metabolism during light exposure in ripe 'red delicious' apples. Both treatments led to improved anthocyanins biosynthesis in detached mature apples, while the NPs-TiO had less impact on the fruit firmness, TSS, TA, and TSS/TA ratio. Furthermore, the effects of both treatments on the expression of anthocyanin-related enzymes and transcription factors in the apple peel were evaluated at the gene level.

View Article and Find Full Text PDF

Although brassinolide (BR) and jasmonic acid (JA) play essential roles in the regulation of cold stress responses, the molecular basis of their crosstalk remains elusive. Here, we show a key component of BR signaling in apple (Malus × domestica), BR INSENSITIVE1 (BRI1)-EMS-SUPPRESSOR1 (BES1)-INTERACTING MYC-LIKE PROTEIN1 (MdBIM1), increases cold tolerance by directly activating expression of C-REPEAT BINDING FACTOR1 (MdCBF1) and forming a complex with C-REPEAT BINDING FACTOR2 (MdCBF2) to enhance MdCBF2-activated transcription of cold-responsive genes. Two repressors of JA signaling, JAZMONATE ZIM-DOMAIN1 (MdJAZ1) and JAZMONATE ZIM-DOMAIN2 (MdJAZ2), interact with MdBIM1 to integrate BR and JA signaling under cold stress.

View Article and Find Full Text PDF