Transcriptomics and Metabolomics Explain the Crisping Mechanisms of Broad Bean-Based Crisping Diets on Nile Tilapia ().

To investigate the crisping mechanism of broad bean-based crisping diets on Nile Tilapia. Four crisping diets were designed to feed 360 fish for 90 days, and multiomics analyses were employed. Our results indicated that the designed crisping diets for Nile tilapia can effectively make tilapia muscle crispy.

Impact of Dietary Variations on Kuruma Shrimp () Assessed through Individual-Based Rearing and Insights into Individual Differences.

This study developed an individual-rearing method to compare the effects of live feed (sandworms ), formulated pellet diets, and a mixture of live feed and formula feed on the Kuruma shrimp , aiming to minimize the influence of non-dietary factors on the growth of , like cannibalism. Results indicated that live feed, with its higher protein, essential amino acids, and fatty acid content, led to significantly better growth and feeding performance in ( < 0.05) compared to pellet diets.

Differential effects of oxytetracycline on detoxification and antioxidant defense in the hepatopancreas and intestine of Chinese mitten crab under cadmium stress.

This study aims to evaluate the effects of oxytetracycline (OTC) on detoxification and oxidative defense in the hepatopancreas and intestine of Chinese mitten crab (Eriocheir sinensis) under cadmium (Cd) stress. The crab was exposed to 0.6 μM Cd, 0.

Effects of Broad Bean Diet on the Growth Performance, Muscle Characteristics, Antioxidant Capacity, and Intestinal Health of Nile Tilapia ().

Four crisping diets were designed to conduct a feeding experiment to investigate the use of broad bean in the formulated feed of Nile tilapia and optimize its use. The growth performance, muscle characteristics, antioxidant capacity, and intestinal health of Nile tilapia with an initial body weight of 617.32 ± 1.

Different effects of continuous and pulsed Benzo[a]pyrene exposure on metabolism and antioxidant defense of large yellow croaker: Depend on exposure duration.

This study aims to compare differential effects of continuous and pulsed BaP exposures on metabolism and antioxidant defense in the liver of large yellow croaker. Fish were subjected to BaP for 4 days and 36 days in three exposure regimes with the same time-averaged concentration of BaP: 4 μg/L BaP continuously, 8 μg/L BaP for 24 h every other day or 16 μg/L BaP for 24 h every 4 days. Our results showed that compared to pulsed BaP exposures, continuous BaP exposure reduced BaP metabolism (CYP1A, CYP3A and AHR transcriptional expressions, GSH content, GSH/GSSG ratio, EROD and GST activities) and antioxidant defense (T-SOD activity) on day 4, resulting to the increases in MDA and PC contents, indicating that continuous BaP exposure induced more severe oxidative damage during the early stage of exposure.

A broad-host-range lytic phage vB_VhaS-R18L as a candidate against vibriosis.

Vibriosis is one of the most common bacterial diseases that cause high rates of mortality and considerable economic losses in aquaculture. Phage therapy has been considered as a promising alternative method to antibiotics in the biocontrol of infectious diseases. Genome sequencing and characterization of the phage candidates are prerequisites before field applications to ensure environmental safety.

Different cold tolerances among three strains of large yellow croaker: related to antioxidant defense and energy metabolism.

The aim of this study was to compare low-temperature tolerances in different strains of large yellow croaker. Dai Qu (DQ), Min-Yue Dong (MY), and Quan Zhou (NZ) strains of large yellow croaker were subjected to cold stress (8.6 °C) for 12 h, 24 h, 48 h, and 96 h.

Treatments of orange-spotted grouper (Epinephelus coioides) against Cryptocaryon irritans with •OH, ClO or HCHO: Survival, physiological and histological response.

Cryptocaryon irritans causes one of the most serious diseases in various wild and cultured marine fish, leading to mass mortality and economic loss. In this study, hydroxyl radical (•OH) solution produced by strong ionization discharge combined with water jet cavitation effect was injected into orange-spotted grouper (Epinephelus coioides) aquaculture tanks for C. irritans control.

Autochthonous Probiotics Alleviate the Adverse Effects of Dietary Histamine in Juvenile Grouper ().

High dose (0.3%) of dietary histamine can cause adverse effects on growth performance, innate immunity, and gut health in juvenile grouper (). In the present study, three autochthonous probiotics ( SE5, sp.

Hypoxic acclimation improves mitochondrial bioenergetic function in large yellow croaker Larimichthys crocea under Cu stress.

The purpose of this study was to investigate how pre-hypoxia exposure affected the mitochondrial structure and bioenergetic function of large yellow croaker in responding to Cu stress. Fish were acclimated to normoxia and 3.0 mg DO L for 48 h, then subjected to 0 and 120 μg Cu L for another 48 h.

Molecular characterization of the interferon regulatory factor (IRF) family and functional analysis of IRF11 in the large yellow croaker (Larimichthys crocea).

Interferon regulatory factors (IRFs) are a family of transcription factors involved in regulating interferon (IFN) responses and immune cell development. A total of 11 IRFs have been identified in teleost fish. Here, a complete repertoire of 11 IRFs (LcIRFs) in the large yellow croaker (Larimichthys crocea) was characterized with the addition of five newly identified members, LcIRF2, LcIRF5, LcIRF6, LcIRF10, and LcIRF11.

Modulation of copper-induced antioxidant defense, Cu transport, and mitophagy by hypoxia in the large yellow croaker (Larimichthys crocea).

This study aimed to investigate the effects of hypoxia on Cu-induced antioxidant defense, Cu transport, and mitophagy in the liver of the large yellow croaker. Fish were exposed to hypoxia (3.0 mg L), Cu (120 μg L), and hypoxia (3.

Pre-hypoxia exposure inhibited copper toxicity by improving energy metabolism, antioxidant defence and mitophagy in the liver of the large yellow croaker Larimichthys crocea.

This study investigated the effects of moderate hypoxia pre-exposure on energy metabolism, antioxidant defence and mitophagy in the liver of the large yellow croaker Larimichthys crocea exposed to Cu. Fish were pre-exposed to either normoxia or hypoxia (~3.0 mg L, 42% O saturation) for 48 h, and subsequently were subjected to either control (without Cu addition) or Cu (168 μg L) under normoxic conditions for another 48 h.

Cu pre-exposure alters antioxidant defense and energy metabolism in large yellow croaker Larimichthys crocea in response to severe hypoxia.

The aim of the present study was to evaluate the effects of Cu pre-exposure on antioxidant defense and energy metabolism in the liver of the large yellow croaker exposed to severe hypoxia. Fish were pre-acclimated to 0 and 30 μg Cu L for 96 h, and subsequently exposed to 7.0 and 1.

Identification and bioactivity of a granulocyte colony-stimulating factor b homologue from large yellow croaker (Larimichthys crocea).

Granulocyte colony-stimulating factor (GCSF) is a pleiotropic cytokine that plays a key role in regulation of hematopoiesis, innate and adaptive immune responses in mammals. However, bioactivity of GCSF in teleost fish remains largely unknown. In this study, a GCSFb homologue from large yellow croaker (Larimichthys crocea) (LcGCSFb) was cloned by RACE-PCR techniques.

Toxicological effects of waterborne Zn on the proximal and distal intestines of large yellow croaker Larimichthys crocea.

The aim of the present study was to compare the differences of Zn-induced antioxidant defense, immunotoxicity and Zn homeostasis between the proximal and distal intestines of the large yellow croaker Larimichthys crocea. Fish were exposed to Zn (2 and 10 mg L) for 96 h. In the proximal intestine, high-concentration Zn increased mortality and oxidative damage, but reactive oxygen species (ROS), lipid peroxidation (LPO), protein carbonylation (PC) levels were not affected by low-concentration Zn, indicating Zn-induced oxidative damage was concentration-dependent.

Essential element Cu and non-essential element Hg exposures have different toxicological effects in the liver of large yellow croaker.

The objective was to compare the different effects of essential element Cu and non-essential element Hg on antioxidant and inflammatory responses in the liver of large yellow croaker Larimichthys crocea. Fish were exposed to Cu stresses (72 and 288 μg L) and Hg stresses (14 and 56 μg L) for 96 h. High-dose Cu increased metallothioneins (MTs) levels and immune defenses in response to elevated reactive oxygen species (ROS), but low-dose Cu had no effect on ROS.

Differential effects of β-glucan on oxidative stress, inflammation and copper transport in two intestinal regions of large yellow croaker Larimichthys crocea under acute copper stress.

The aim of the present study was to evaluate investigate the effects of β-glucan on oxidative stress, inflammation and copper transport in two intestinal regions of large yellow croaker under acute copper stress. Fish were injected with β-glucan at a dose of 0 or 5 mg kg body weight on 6, 4 and 2 days before exposed to 0 and 368 μg Cu L for 48 h. Biochemical indicators (MDA, Cu content, MTs protein levels, Cu/Zn-SOD, CAT and iNOS activities), gene expressions of oxidative stresses (Cu/Zn-SOD, CAT, Nrf2, MTs and MTF-1), inflammatory responses (NF-κB, iNOS, IL-1β, IL-6 and TNF-α) and Cu transporters (ATP7A, ATP7B and CTR1) were determined.

Molecular cloning and bioactivity of an IL-2 homologue in large yellow croaker (Larimichthys crocea).

Interleukin-2 (IL-2), an important immunomodulatory cytokine, plays a crucial role in promoting the proliferation, activation and differentiation of T cells. Here, the cDNA of an IL-2 homologue (LcIL-2) in large yellow croaker (Larimichthys crocea) was cloned by RACE-PCR techniques. The open reading frame (ORF) of LcIL-2 gene is 426 bp long and encoded a precursor protein of 141 amino acids (aa), with a 20-aa signal peptide and a 121-aa mature peptide containing two putative N-glycosylation sites at Asn and Asn.

Abrupt salinity stress induces oxidative stress via the Nrf2-Keap1 signaling pathway in large yellow croaker Pseudosciaena crocea.

The aim of the present study was to evaluate the effects of abrupt salinity stress (12, 26 (control), and 40) on lipid peroxidation, activities and mRNA levels of antioxidant enzymes (Cu/Zn-SOD, CAT, GPx, and GR), and gene expression of the Nrf2-Keap1 signaling molecules at different times (6, 12, 24, and 48 h) in the liver of large yellow croaker Pseudosciaena crocea. The results showed that lipid peroxidation was sharply reduced at 6 h and increased at 12 h before returning to control levels in the hypo-salinity group. Similarly, lipid peroxidation was significantly decreased at 6 h followed by a sharp increase towards the end of the exposure in the hyper-salinity group.

Molecular characterization and evolution analysis of five interleukin-17 receptor genes in large yellow croaker Larimichthys crocea.

Interleukin-17s (IL-17s) play critical roles in inflammatory response and host defense against extracellular pathogens. IL-17s induce the immune response signaling through the specific IL-17 receptors (IL-17Rs) that consist of five members (IL-17RA to E). In the present work, we have identified the five IL-17R orthologs (LycIL-17Rs) from large yellow croaker Larimichthys crocea.

Effects of β-glucan on ROS production and energy metabolism in yellow croaker (Pseudosciaena crocea) under acute hypoxic stress.

The aim of the present study was to evaluate the effect of β-glucan on acute hypoxia-induced oxidative stress and the changes in energy metabolism by determining ROS production, activities and mRNA levels of energy metabolism enzyme (PK, F-ATPase, SDH and MDH), and in gene expression of HIF-1α in the liver of large yellow croaker. Fish were injected with β-glucan at a dose of 0 or 5 mg kg(-1) body weight on 6, 4 and 2 days before exposed to 1.5 and 7.

Molecular and functional identification of three interleukin-17A/F (IL-17A/F) homologues in large yellow croaker (Larimichthys crocea).

The interleukin-17 (IL-17) cytokine family plays a central role in the coordination of inflammatory responses. In fish species, three genes that have a similar homology to both IL-17A and IL-17F were designated IL-17A/F1, 2, and 3. In this study, we identified three IL-17A/F homologues (LycIL-17A/F1, 2, and 3) from large yellow croaker (Larimichthys crocea).

Pleomorphobacterium xiamenense gen. nov., sp. nov., a moderate thermophile isolated from a terrestrial hot spring.

An aerobic, motile, moderately thermophilic rod, designated strain CLW(T), was isolated from a terrestrial hot spring in an exposition garden in Xiamen City, Fujian Province, the People's Republic of China. Strain CLW(T) formed beige, dry colonies on solid 2216E medium and flocks in liquid medium. Cells were Gram-stain-negative, short rods (1.