J Clin Microbiol
January 2024
Spacer oligonucleotide typing (spoligotyping), the first-line genotyping assay for (MTB), plays a fundamental role in the investigation of its epidemiology and evolution. In this study, we established a single-tube spoligotyping assay using MeltArray, a highly multiplex polymerase chain reaction (PCR) approach that runs on a real-time PCR thermocycler. The MeltArray protocol included an internal positive control, , to indicate the abundance of MTB via the quantification cycle and 43 spacers to identify the spoligotype via melting curve analysis.
View Article and Find Full Text PDF© LitMetric 2025. All rights reserved.