Inflammation status of rabbit carotid artery model endothelium during intermittent hypoxia exposure and its relationship with leptin.

Objectives: To develop an intermittent hypoxia/reoxygenation (IH/ROX) rabbit carotid artery model and then investigate the inflammation status of rabbit carotid artery endothelium after IH exposure and its relationship with leptin.

Materials And Methods: After anesthetization, rabbit's right common carotid artery was cleared of surrounding tissue with anatomic microscope, cannulated to its distal part and the proximal part was ligated. Preparations were challenged by changing the PO(2) of the gas mixture equilibrating the perfusate.

[Comparative genomic hybridization: the profile of chromosomal imbalances in rhabdomyosarcoma].

Objective: To characterize the profile of chromosomal imbalances of rhabdomyosarcoma(RMS).

Methods: Comparative genomic hybridization (CGH) was used to investigate genomic imbalances in 25 cases of primary RMS including 10 cases of alveolar rhabdomyosarcoma (ARM), 12 cases of embryonic rhabdomyosarcoma (ERMS), 3 cases of polymorphic rhabdomyosarcoma (PRMS) and 2 RMS cell lines (A240 originated from ARMS and RD from PRMS), with correlation to histological type, pathologic grading, clinical staging, gender and age, respectively.

Results: All twenty-five rhabdomyosarcomas showed evidence of increased or decreased DNA sequence copy numbers involving one or more regions of the genome.

[Carotid body inflammation and carotid sinus nerve afferent activity after intermittent hypoxia exposure of various frequencies in rabbits].

Objective: To explore the inflammatory reactions, endothelin level and carotid sinus nerve (CSN) afferent activity of carotid body (CB) after intermittent hypoxia/reoxygenation (IH/ROX) exposure of various frequencies in rabbits.

Methods: Forty-nine male adult New Zealand white rabbits (2.5-3.

[Sorting of spermatogonial stem cells in mice].

Objective: To search for an effective method for enriching spermatogonial stem cells in mice.

Methods: Bilateral artificial cryptorchidism was performed on 20 six-week old male Kunming mice. Three months after the operation, the testes were removed and single cell suspension prepared by two-step enzyme digestion.

[Antitumor effect of alcohol extract from Sesamum indicum flower on S180 and H22 experimental tumor].

Objective: To investigate the antineoplasm effect of extract from Sesamum indicum L. flower.

Method: Observing the effects of alcohol extract from Sesamum indicum flower on tumor growth in sarcoma 180 (S180) and Heps 22 (H22) tumorigenic mouse, and on weight of immune organs.

Caspases promoted DADAG-induced apoptosis in human leukemia HL-60 cells.

Aim: To investigate the roles of caspases in diacetyldianhydrogalactitol (DADAG)-induced apoptosis in human leukemia HL-60 cells.

Methods: Inhibition of proliferation was measured by MTT assay. DADAG-induced apoptosis in HL-60 cells was observed by electron microscopy, flow cytometry, and DNA fragmentation assay.