Abundance of Transgene Transcript Variants Associated with Somatically Active Transgenic from Multiple T-DNA Integration Sites in Maize.

, a novel type of mysterious DNA transposons discovered computationally prior to bench work confirmation, are components ubiquitous in most sequenced genomes of various eukaryotes, including plants, animals, and fungi. There is a paucity of empirical evidence to elucidate the mechanism of transposition in plants. Here, by constructing several artificial defective () reporter systems, we aim to identify the autonomous () in maize genetically and to demonstrate the transposition and repair mechanisms of upon the excision in maize.

Cloning of Maize Transposon into Reveals the Polychromatic Sequence Landscape of Refractorily Propagated Plasmids.

, the founder member of the superfamily and its MURA transcripts, has been identified as toxic sequences to (), which heavily hindered the elucidation of the biochemical features of MURA transposase and confined the broader application of the system in other organisms. To harness less constrained systems as alternatives, we attempted to clone and two recently isolated autonomous -like elements () from maize, respectively. Their full-length transcripts and genomic copies are successfully cloned when the incubation time for bacteria to recover from heat shock is extended appropriately prior to plating.

Progress on Mutator superfamily.

Transposable elements (TEs) are fragments of DNA sequence, which can mobile from one locus to another within a genome, often replication in the process. Occupying the main component of the genome, TEs can affect the structure and function of gene and/or genome in a variety of ways, and play an important role in the evolution of the host. Based on the transposition intermediate, eukaryotic TEs can be divided into two classes.