A 3D microfluidic paper-based electrochemical immunodevice (μ-PEID) for simultaneous sensitive detection of two tumor biomarkers was fabricated. A nanoporous Pt particle modified paper working electrode (NPPt-PWE) was used as the matrix and metal ions loaded on the l-cysteine capped flower-like Au nanoparticles (Au-Cys) were used as a signal amplification label. The NPPt-PWE was constructed through the growth of a NPPt layer on the back of the paper working electrode to improve electron conduction.
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