Genome-wide analysis of Class III peroxidase (PRX) family core genes and functional mechanism of GhPRXR1-A for seed development in Gossypium hirsutum.

Class III peroxidases (PRXs) play critical roles in plant growth and development by oxidizing various substrates with HO. Although many PRXs have been identified and their roles in biotic and abiotic stress responses have extensively investigated in plants. However, functional mechanisms of PRXs in seed development remain poorly understood.

[VEGF short hairpin RNA expression plasmid blocks VEGF expression in rat vascular endothelial cell and inhibits rat corneal neovascularization].

Objective: To investigate RNA interference in rat corneal neovascularization and vascular endothelial cells of vascular endothelial growth factor expression and its mechanism.

Methods: In empirical study short hairpin RNA (shRNA) targeting VEGF mRNA was designed and synthesized and VEGF shRNA eukaryotic plasmids were transfected into EC with Lipofectamine 2000 transfection system. Expression of VEGF mRNA and protein in transfected cells was detected by RT-PCR and Western blot.