Publications by authors named "Chunling Chang"

The biological degradation of plant residues in the soil or on the soil surface is an integral part of the natural life cycle of annual plants and does not have adverse effects on the environment. Crop straw is characterized by a complex structure and exhibits stability and resistance to rapid microbial decomposition. In this study, we conducted a microcosm experiment to investigate the dynamic succession of the soil microbial community and the functional characteristics associated with lignocellulose-degrading pathways.

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A fundamental question in ecology is which species will prevail over others amid changes in both environmental mean conditions and their variability. Although the widely accepted fluctuating resource hypothesis predicts that increases in mean resource availability and variability therein will promote nonnative plant invasion, it remains unclear to what extent these effects might be mediated by soil microbes. We grew eight invasive nonnative plant species as target plants in pot-mesocosms planted with five different synthetic native communities as competitors, and assigned them to eight combinations of two nutrient-fluctuation (constant vs.

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Two-dimensional fractal topologies featuring (scaling) self-similarity, dense set of Bragg (diffraction) peaks, and inherent rotation symmetry, which are not achievable with regular grid-matrix geometries, exhibit optical robustness against structural damage and noise immunity of optical transmission paths. In this work, we numerically and experimentally demonstrate phase holograms using fractal plane-divisions. By taking advantage of the symmetries of the fractal topology, we propose numerical algorithms to design the fractal holograms.

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In this article, H control for stochastic singular time-varying delay systems under arbitrarily variable samplings is addressed via designing a sampled-data controller. The first and foremost, a novel time-dependent discontinuous Lyapunov-Krasovskii (L-K) functional is built, which takes good advantage of the factual sampling pattern's available properties. Then, based on the refined input delay method by utilizing the constructed time-dependent L-K functional, the free-weighting matrix method, and the auxiliary vector function approach are adopted to develop conditions ensuring the stochastic admissibility for the studied stochastic singular systems with time-varying delays.

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Soil physicochemical properties and fungal communities are pivotal factors for continuous cropping of American ginseng (Panax quinquefolium L.). However, the response of soil physicochemical properties and fungal communities to replant disease of American ginseng has not yet been studied.

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The rhizomicrobial community is influenced by plant genotype. However, the potential differences in the co-assembly of bacterial and fungal communities between parental lines and different generations of rice progenies have not been examined. Here we compared the bacterial and fungal communities in the rhizomicrobiomes of female parent wild rice; male parent cultivated rice; their F1 progeny; and the F2, F3 and F4 self-crossing generations.

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Little known the connections between soybeans mitogen-activated protein kinase () gene expression and the rhizomicrobiome upon invasion of the root pathogen . To address this lack of knowledge, we assessed the rhizomicrobiome and root transcriptome sequencing of wild and cultivated soybean during the invasion of . Results indicated infection enriched spp.

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The rhizomicrobiome helps the host plant to better adapt to environmental stresses. In contrast, plant-derived metabolic substances, including phytohormones, play an active role in structuring rhizomicrobiome. Although strigolactones (SLs), a group of phytohormones, serve as potential rhizosphere signaling molecules, their contributions in shaping the rice (Oryza sativa) rhizomicrobiome remain elusive.

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This study presents evidence that strigolactones (SLs) promote defense against devastating rice blast fungal pathogen Magnaporthe oryzae. Impairment in either SL-biosynthetic dwarf17 (d17) or -signaling (d14) led to increased susceptibility towards M. oryzae.

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The rhizospheric microbiome appears to be one of the key determinants of plant health and productivity. In this study, to understand the assembly process of the rhizospheric microbiome, the effects of different sites, soils and plants on the rhizospheric microbiome were compared and examined using high-throughput sequencing. A series of comparisons of rhizospheric microbiomes were conducted using two plants (wild rice (Oryza rufipogon Griff.

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Background: Rice, which serves as a staple food for more than half of the world's population, is very susceptible to the pathogenic fungus, Magnaporthe oryzae. However, common wild rice (Oryza rufipogon), which is the ancestor of Asian cultivated rice (O. sativa), has significant potential as a genetic source of resistance to M.

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In this study, type I collagen membranes were prepared using oligomeric proanthocyanidins (OPCs) as the cross-linking agent. The fabricated materials were evaluated to be applied as guided tissue regeneration membranes for periodontal defects. The mechanical strength of the cross-linked collagen membranes, namely OPCs-Col films, using different concentrations of OPCs ranged from 30 to 60 kPa.

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The integration of III-V and Si multi-junction solar cells as photovoltaic devices has been studied in order to achieve high photovoltaic conversion efficiency. However, large differences in the coefficients of thermal expansion and the lattice parameters of GaAs, Si, and InGaAs have made it difficult to obtain high-efficiency solar cells grown as epilayers on Si and InP substrates. In this paper, two types of devices, including GaInP/GaAs stacked on Si (GaInP/GaAs//Si) and GaInP/GaAs stacked on InGaAs (GaInP/GaAs//InGaAs), are fabricated via mechanical stacking and wire bonding technologies.

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Cultivated soybean (Glycine max) was derived from the wild soybean (Glycine soja), which has genetic resources that can be critically important for improving plant stress resistance. However, little information is available pertaining to the molecular and physiochemical comparison between the cultivated and wild soybeans in response to the pathogenic Fusarium oxysporum Schltdl. In this study, we first used comparative phenotypic and paraffin section analyses to indicate that wild soybean is indeed more resistant to F.

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Background: Magnaporthe oryzae, the causal fungus of rice blast disease, negatively impacts global rice production. Wild rice (Oryza rufipogon), a relative of cultivated rice (O. sativa), possesses unique attributes that enable it to resist pathogen invasion.

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Plant pathogens represent a huge threat to world food security, affecting both crop production and quality. Although significant progress has been made in improving plant immunity by expressing key, defense-related genes and proteins from different species in transgenic crops, a challenge remains for molecular breeders and biotechnologists to successfully engineer elite, transgenic crop varieties with improved resistance against critical plant pathogens. Upon pathogen attack, including infection of rice (Oryza sativa) by Magnaporthe oryzae, host plants initiate a complex defense response at molecular, biochemical and physiological levels.

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Article Synopsis
  • Higher GC-content in DNA leads to higher melting temperatures and greater heat absorption compared to regions with lower GC-content.
  • * Calorimetric melting profiles (cDMCs) and differential melting curves (DMCs) do not provide equivalent data, prompting the development of expressions to convert between them for DNA sequences.
  • * This new method allows for the determination of the thermodynamic melting temperature of natural DNAs using optical DMCs, eliminating the need for calorimetric experiments.
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  • The Poland-Fixman-Freire formalism was adapted to analyze calorimetric DNA melting profiles for plasmid pBR 322 and long random DNA sequences.
  • The study investigated how the difference in enthalpies (HGC - HAT) between AT and GC base pairs affected the calorimetric melting profiles, revealing significant changes due to this difference.
  • It was found that while the normalized profiles show slight changes, both the average relative deviation and the difference in melting temperatures between calorimetric profiles and normalized data increase with the temperature range of the helix-coil transition.
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  • The temperature and shape of DNA's helix-coil transition can be affected by various factors, leading to multi-peak differential melting curves (DMCs) that complicate defining melting temperature (Tm) and melting width (ΔT).
  • There's no consensus on how to best define Tm and ΔT, with changes in these values indicating instability in DMC shapes and variations in DNA stability.
  • Experiments and computer modeling showed that using Tm as the average temperature of the helix-coil transition and ΔT based on temperature deviation offers a consistent way to characterize multi-peak DMCs, aligning closely with thermodynamic principles.
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Molecular combing and flow-induced stretching are the most commonly used methods to immobilize and stretch DNA molecules. While both approaches require functionalization steps for the substrate surface and the molecules, conventionally the former does not take advantage of, as the latter, the versatility of microfluidics regarding robustness, buffer exchange capability, and molecule manipulation using external forces for single molecule studies. Here, we demonstrate a simple one-step combing process involving only low-pressure oxygen (O2) plasma modified polysilsesquioxane (PSQ) polymer layer to facilitate both room temperature microfluidic device bonding and immobilization of stretched single DNA molecules without molecular functionalization step.

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Article Synopsis
  • Cisplatin exhibits antitumor properties by forming covalent bonds with DNA, leading to decreased thermal stability, while transplatin, its inactive counterpart, actually increases thermal stability.
  • Differential scanning calorimetry (DSC) and computer modeling reveal that both compounds reduce the enthalpy and entropy during DNA transitions, with transplatin having a more pronounced effect.
  • The differences in thermal stability between cisplatin and transplatin complexes are primarily due to their distinct intrastrand crosslink thermodynamics, which are crucial for cisplatin's antitumor activity.
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  • Cisplatin, an antitumor drug, disrupts DNA structure by forming various adducts that lead to a significant decrease in melting enthalpy, indicating a structural impact on DNA, especially in a neutral medium.
  • In alkaline conditions, the negative thermal effect (δT(m)) of cisplatin is enhanced, while its inactive analogs, transplatin and Pt(dien)Cl, also cause distortions in DNA but produce a positive thermal effect.
  • Cisplatin's intrastrand crosslinks show a much greater thermal destabilization compared to its monofunctional adducts, highlighting the relationship between these crosslinks and its potent antitumor activity.
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Article Synopsis
  • - The study focuses on the thermodynamics of long DNA interstrand crosslinks (ICLs) formed by anticancer drugs, which have not been thoroughly investigated compared to shorter DNA duplexes.
  • - Through computer modeling, researchers found that ICLs can significantly alter the melting temperature of DNA, with changes ranging from -17 to +47°C, depending on specific free energy factors, while being only slightly influenced by DNA sequence and GC content.
  • - The research proposes methods for understanding the impact of different crosslinking agents on DNA stability, including a comparative approach for evaluating the thermal effects and structural alterations caused by ICLs.
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Aim Of Study: To evaluate the potential expectorant and antitussive activity of a traditional Chinese medicine.

Materials And Methods: The water extract and four fractions of the aerial part of Reineckia carnea were orally administrated to coughing mice induced by ammonium hydroxide and mice injected with phenol red, respectively, to investigate their medification effect on coughing and mucus secretion.

Results And Discussion: 90% Ethanol fraction significantly lengthened the latent period of cough and decreased cough frequency caused by ammonium hydroxide at the dose of 0.

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Differential scanning calorimetry, circular dichroism spectroscopy, nuclear magnetic resonance spectroscopy, and numerical simulations were used to study the thermostability of the N-terminal RNA-binding domain (RBD) of the SARS-CoV nucleocapsid protein. The transition temperature of the RBD in a mixing buffer, composed of glycine, sodium acetate, and sodium phosphate with 100 mM sodium chloride, at pH 6.8, determined by differential scanning calorimetry and circular dichroism, is 48.

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