Expression of β-Glucosidases from the Yak Rumen in Lactic Acid Bacteria: A Genetic Engineering Approach.

β-glucosidase derived from microorganisms has wide industrial applications. In order to generate genetically engineered bacteria with high-efficiency β-glucosidase, in this study two subunits ( and ) of β-glucosidase obtained from the yak rumen were expressed as independent proteins and fused proteins in lactic acid bacteria ( NZ9000). The engineered strains NZ9000/pMG36e-usp45-, NZ9000/pMG36e-usp45-, NZ9000/pMG36e-usp45--usp45- were successfully constructed.