Targeting the smooth muscle cell Keap1-Nrf2-GSDMD-pyroptosis axis by cryptotanshinone prevents abdominal aortic aneurysm formation.

: Abdominal aortic aneurysm (AAA) is an inflammatory, fatal aortic disease that currently lacks any effective drugs. Cryptotanshinone (CTS) is a prominent and inexpensive bioactive substance derived from , a well-known medicinal herb for treating cardiovascular diseases through its potent anti-inflammatory properties. Nevertheless, the therapeutic effect of CTS on AAA formation remains unknown.

Ethyl Lithospermate Reduces Lipopolysaccharide-Induced Inflammation through Inhibiting NF-κB and STAT3 Pathways in RAW 264.7 Cells and Zebrafish.

Objective: To explore the anti-inflammatory effects of ethyl lithospermate in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine-derived macrophages and zebrafish, and its underlying mechanisms.

Methods: 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assays were performed to investigate the toxicity of ethyl lithospermate at different concentrations (12.

Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling.

Ethnopharmacological Relevance: Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality. Inflammation is closely related to the occurrence of CVDs. As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.

Sequencing by avidity enables high accuracy with low reagent consumption.

We present avidity sequencing, a sequencing chemistry that separately optimizes the processes of stepping along a DNA template and that of identifying each nucleotide within the template. Nucleotide identification uses multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer-nucleotide complexes bound to clonal copies of DNA targets. These polymer-nucleotide substrates, termed avidites, decrease the required concentration of reporting nucleotides from micromolar to nanomolar and yield negligible dissociation rates.

Response of Soil Detachment Rate to Sediment Load and Model Examination: A Key Process Simulation of Rill Erosion on Steep Loessial Hillslopes.

The rate of soil detachment by water flow indicates soil erosion intensity directly. The exact relation between soil detachment rate and actual sediment load in water flow, however, is still unclear, and the existing relationships have not been adequately tested. The aims of the present study were to investigate the response of soil detachment rate to sediment load using rill flume data with loessial soil and to quantitatively examine the soil detachment equations in the WEPP and EUROSEM soil erosion models.

Modeling study on the roles of the deposition and transport of PM in air quality changes over central-eastern China.

The role of PM (particles with aerodynamic diameters ≤ 2.5 µm) deposition in air quality changes over China remains unclear. By using the three-year (2013, 2015, and 2017) simulation results of the WRF/CUACE v1.

[Zebrafish's Circular RNAs].

Circular RNAs (circRNAs),a group of highly conserved small RNAs,are characterized by a closed circular structure from precursor linear RNA through reverse splicing.They are powerful regulators of the physiological and pathological processes in organisms at different development stages.Zebrafish can be used for the high-throughput drug screening with low cost.

Ethyl Acetate Fraction of Induces Apoptosis via JNK/Nur77 Pathway in Hepatocellular Carcinoma Cells.

Background: Hepatocellular carcinoma (HCC) is characterized by poor diagnosis and high mortality. Novel and efficient therapeutic agents are urgently needed for the treatment. (HDW) is used to treat cancers, especially HCC in China.

Confined catalysis of MOF-818 nanozyme and colorimetric aptasensing for cardiac troponin I.

Understanding the catalytic performance of nanozymes assembled in confined environment is an interesting topic. Herein, a three-dimensional nanozyme-catalytic nanoreactor was constructed by confining MOF-818 nanozyme in the pore of macroporous tungsten trioxide (p-WO). The catalytic activity of MOF-818 assembled in-situ for the oxidation of 3,5-Di-tert-butylcatechol (3,5-DTBC) could be regulated by changing the pore size of p-WO.

Research on Key Competency Elements of an Excellent Front-Line Health Inspector.

In order to find out the competency elements of excellent front-line health inspector, and to improve the comprehensive quality and law enforcement level, we conducted this study. Firstly, key Behavior Event Interview (BEI) was used to find out the competency elements of front-line inspector. From May to September in 2020, 7 health inspectors engaged in front-line inspection and law enforcement work in the Pudong New Area Health Inspection agency were interviewed by using the method of key Behavior Event Interview (BEI).

Effect of vegetation seasonal cycle alterations to aerosol dry deposition on PM concentrations in China.

The effect of vegetation seasonal cycle alterations to aerosol dry deposition on PM concentrations (hereafter referred as the VSC effect) in China was investigated using a numerical modelling system (WRF/CUACE). Two simulation experiments using the vegetation parameters in particle dry deposition schemes typical for January and July revealed an absolute increase in surface PM concentrations of about 2.4 μg/m and a 5.

Photoelectrochemical aptasensing for thrombin based on exonuclease III-assisted recycling signal amplification and nanoceria enzymatic strategy.

In the present work, a capture DNA (c-DNA) was immobilized on the TNA/g-CN to develop a sensitive and selective TNA/g-CN/c-DNA photoelectrochemical aptasensor for determining thrombin. With the aid of the specific recognition of anti-thrombin aptamer towards thrombin, ingenious design of hairpin DNA, and exonuclease III-assisted recycling signal amplification, more nanoceria could be assembled on the TNA/g-CN/c-DNA to form TNA/g-CN/nanoceria in the presence of thrombin. Due to the oxidase-mimic catalytic efficiency of nanoceria and the oxygen consumption for glucose oxidation, the photoexcited electrons at the conduction band of g-CN could be well transferred to that of TNA under visible light irradiation, resulting in the increase of the photocurrent of TNA/g-CN/nanoceria, and the increase value of photocurrent had a linear relationship with the concentration of thrombin under the optimal conditions.

Measurement of Human Resource Demand of Medical Institution Inspection Based on Task Analysis.

Purpose: To estimate the human resource demand of health inspection agencies to carry out the medical institution inspection function, and to provide the basis for the rational allocation of health inspection human resources.

Methods: Through policy documents review, inquiry of data from national health inspection report, Shanghai Health Inspection core business system, the basic data were collected. Based on the task analysis, the inspection functions of health inspection agency were investigated.

A new parameterization of uptake coefficients for heterogeneous reactions on multi-component atmospheric aerosols.

Based on laboratory studies and field observations, a new parameterization of uptake coefficients for heterogeneous reactions on multi-component aerosols is developed in this work. The equivalent ratio (ER) of inorganic aerosol is used to establish the quantitative relationship between the heterogeneous uptake coefficients and the composition of aerosols. Incorporating the new ER-dependent scheme, the WRF-CUACE model has been applied to simulate sulfate mass concentrations during December 2017 in the Beijing-Tianjin-Hebei region and evaluate the role of aerosol chemical components played in the sulfate formation.

Self-powered aptasensing for prostate specific antigen based on a membraneless photoelectrochemical fuel cell.

A self-powered aptasensor for prostate specific antigen (PSA) based on a membraneless photoelectrochemical fuel cell (PEFC) with double photoelectrodes was constructed, in which, PSA-binding aptamer was electrostatically immobilized on the KOH-doped g-CN modified TiO nanotube arrays (TNA/A-g-CN/aptamer), which was used as a photoanode, and Fe-doped CuBiO modified indium doped tin oxide (ITO) substrate (ITO/CBFeO) was used as a photocathode. Under visible light irradiation, glucose was photocatalytically oxidized by A-g-CN and generated HO in situ, which was used as the electron acceptor for ITO/CBFeO photocathode, thus producing a high cell output response with a maximum output power of 133.5 μW cm and an open circuit potential of 0.

Photoelectrochemical determination of alkaline phosphatase activity based on a photo-excited electron transfer strategy.

A sensitive photoelectrochemical (PEC) biosensor for determination of alkaline phosphatase (ALP) activity was constructed based on a photo-excited electron transfer strategy. Immobilization of CdTe quantum dots (QDs) on TiO nanotube arrays (TNAs), addition of iron (III) and adenosine triphosphate (ATP) in turn can effectively adjust the photocurrent response of TNAs under visible light irradiation due to a photo-excited electron transfer process, and alkaline phosphatase (ALP) activity can be determined for its catalysis toward dephosphorylation of ATP. The preparation of CdTe QDs, construction of TNA/QD PEC biosensor and the mechanism of photo-excited electron transfer are investigated in the present work.

Temporal and spatial variations in sand and dust storm events in East Asia from 2007 to 2016: Relationships with surface conditions and climate change.

We analyzed the frequency and intensity of sand and dust storms (SDSs) in East Asia from 2007 to 2016 using observational data from ground stations, numerical modeling, and vegetation indices obtained from both satellite and reanalysis data. The relationships of SDSs with surface conditions and the synoptic circulation pattern were also analyzed. The statistical analyses demonstrated that the number and intensity of SDS events recorded in spring during 2007 to 2016 showed a decreasing trend.

Development of a New Marker System for Identification of and .

Lemnaceae (commonly called duckweed) is an aquatic plant ideal for quantitative analysis in plant sciences. Several species of this family represent the smallest and fastest growing flowering plants. Different ecotypes of the same species vary in their biochemical and physiological properties.

Modeling the interplay between the HIF-1 and p53 pathways in hypoxia.

Both the hypoxia-inducible factor-1 (HIF-1) and tumor suppressor p53 are involved in the cellular response to hypoxia. How the two transcription factors interact to determine cell fates is less well understood. Here, we developed a network model to characterize crosstalk between the HIF-1 and p53 pathways, taking into account that HIF-1α and p53 are targeted for proteasomal degradation by Mdm2 and compete for binding to limiting co-activator p300.

DNA sequencing using polymerase substrate-binding kinetics.

Next-generation sequencing (NGS) has transformed genomic research by decreasing the cost of sequencing. However, whole-genome sequencing is still costly and complex for diagnostics purposes. In the clinical space, targeted sequencing has the advantage of allowing researchers to focus on specific genes of interest.

Involvement of miR-605 and miR-34a in the DNA damage response promotes apoptosis induction.

MicroRNAs are key regulators of gene expression at the posttranscriptional level. In this study, we focus on miR-605 and miR-34a, which are direct transcriptional targets of p53 and in turn enhance its tumor suppressor function by acting upstream and downstream of it, respectively. miR-605 promotes p53 activation by repressing the expression of mdm2, whereas miR-34a promotes p53-dependent apoptosis by suppressing the expression of antiapoptotic genes such as bcl-2.

Structural elucidation of polysaccharide fractions from brown seaweed Sargassum pallidum.

The structural characteristics of two purified fractions of polysaccharides from Sargassum pallidum (SPS) were investigated in the present study. As results, the molecular weights of the two polysaccharide fractions, SPS-3-1 and SPS-3-2, were determined to be 5.87 and 7.

Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum.

Supercritical CO2 extraction, ultrasonic-aid extraction and membrane separation technology were applied to prepare Sargassum pallidum polysaccharides (SP). Three main fractions, SP-1, SP-2 and SP-3, were obtained by membranes of 1.0×10(-4)mm pore size and normal molecular-weight cut-off of 50kDa.

Investigation of pollution characteristics of polychlorinated biphenyls in the typical drinking water sources in Jiangsu Province, China.

Concentration and composition of polychlorinated biphenyls (PCBs) in the typical drinking water sources in Jiangsu Province were studied by scene investigation and physical and chemical analyses as well. Total amount of PCBs in some surface water and surface microlayers exceeded the standard (20 ng/l) in the "Environmental Quality Standard of Surface Water". There were less PCBs in suspended substances and bottom mud.

Cue combination and color edge detection in natural scenes.

Biological vision systems are adept at combining cues to maximize the reliability of object boundary detection, but given a set of co-localized edge detectors operating on different sensory channels, how should their responses be combined to compute overall edge probability? To approach this question, we collected joint responses of red-green and blue-yellow edge detectors both ON- and OFF-edges using a human-labeled image database as ground truth (D. Martin, C. Fowlkes, D.