Label-Free Three-Dimensional Morphological Characterization of Cell Death Using Holographic Tomography.

This study presents a novel label-free approach for characterizing cell death states, eliminating the need for complex molecular labeling that may yield artificial or ambiguous results due to technical limitations in microscope resolution. The proposed holographic tomography technique offers a label-free avenue for capturing precise three-dimensional (3D) refractive index morphologies of cells and directly analyzing cellular parameters like area, height, volume, and nucleus/cytoplasm ratio within the 3D cellular model. We showcase holographic tomography results illustrating various cell death types and elucidate distinctive refractive index correlations with specific cell morphologies complemented by biochemical assays to verify cell death states.

Influence of noise-reduction techniques in sparse-data sample rotation tomographic imaging.

Data acquisition and processing is a critical issue for high-speed applications, especially in three-dimensional live cell imaging and analysis. This paper focuses on sparse-data sample rotation tomographic reconstruction and analysis with several noise-reduction techniques. For the sample rotation experiments, a live Candida rugosa sample is used and controlled by holographic optical tweezers, and the transmitted complex wavefronts of the sample are recorded with digital holographic microscopy.

Combining Whispering-Gallery Mode Optical Biosensors with Microfluidics for Real-Time Detection of Protein Secretion from Living Cells in Complex Media.

The noninvasive monitoring of protein secretion of cells responding to drug treatment is an effective and essential tool in latest drug development and for cytotoxicity assays. In this work, a surface functionalization method is demonstrated for specific detection of protein released from cells and a platform that integrates highly sensitive optical devices, called whispering-gallery mode biosensors, with precise microfluidics control to achieve label-free and real-time detection. Cell biomarker release is measured in real time and with nanomolar sensitivity.

Electrostatic Assist of Liquid Transfer between Flat Surfaces.

Transfer of liquid from one surface to another plays a vital role in printing processes. During liquid transfer, a liquid bridge is formed and subjected to substantial extension but incomplete liquid transfer can produce defects that are detrimental to the operation of printed electronic devices. One strategy for minimizing these defects is to apply an electric field, a technique known as electrostatic assist (ESA).

Stretching liquid bridges with moving contact lines: comparison of liquid-transfer predictions and experiments.

Transfer of liquid from one surface to another plays a key role in printing processes. During liquid transfer, a liquid bridge is formed and then undergoes significant extensional motion while its contact lines are free to move on the bounding solid surfaces. In this work, we develop one-dimensional (1D) slender-jet and two-dimensional (2D) axisymmetric models of this phenomenon and compare the resulting predictions with previously published experimental data.

Observation of the rose petal effect over single- and dual-scale roughness surfaces.

Rose petals exhibit superhydrophobicity with strong adhesion to pin water drops, known as the 'petal effect.' It is generally believed that the petal effect is attributed to dual-scale roughness, that is, the surface possesses both a nanostructure and a microstructure (Feng et al 2008 Langmuir 24 4114). In this study, we demonstrate that the dual-scale roughness is not a necessary condition for a surface of the petal effect.