Lithocholic acid (LCA) is accumulated in mammals during calorie restriction and it can activate AMP-activated protein kinase (AMPK) to slow down ageing. However, the molecular details of how LCA activates AMPK and induces these biological effects are unclear. Here we show that LCA enhances the activity of sirtuins to deacetylate and subsequently inhibit vacuolar H-ATPase (v-ATPase), which leads to AMPK activation through the lysosomal glucose-sensing pathway.
View Article and Find Full Text PDFIntroduction: Psoriasis is a chronic inflammatory skin disease that can present in various phenotypes, with the most common form being plaque psoriasis. Currently, no type of psoriasis can be cured, and existing treatment options are associated with certain safety concerns. In recent years, traditional Chinese medicine has achieved great results in treating psoriasis.
View Article and Find Full Text PDFPurpose: This study aimed to develop and evaluate a machine learning model combining clinical, radiomics, and deep learning features derived from PET/CT imaging to predict lymph node metastasis (LNM) in patients with non-small cell lung cancer (NSCLC). The model's interpretability was enhanced using Shapley additive explanations (SHAP).
Methods: A total of 248 NSCLC patients who underwent preoperative PET/CT scans were included and divided into training, test, and external validation sets.
Black phosphorus quantum dots (BPQDs) are believed to have broad prospects for application. Obesity has garnered significant attention, but the association between BPQDs and lipid metabolism has not been thoroughly investigated. Mice were orally exposed to BPQDs at doses of 0.
View Article and Find Full Text PDFBlack phosphorus quantum dots (BPQDs) have been used as the nano-carrier in the field of biomedicine due to their excellent electronic conductivity, optical and thermoelectric properties. However, it is still lack of evaluation of the safety of BPQDs, specifically on the effects and mechanisms of BPQDs on heart. In this study, the specific pathogen-free male mice were orally administered with different doses of BPQDs (0.
View Article and Find Full Text PDFRural drinking water often suffers from unpleasant odors like dimethyl sulfide (DMDS) and dimethyl trisulfide (DMTS) due to poor raw water quality and limited treatment options. This study introduces durable chitosan/polyvinyl alcohol (PVA) biochar aerogels-supported bioflims in ultrafiltration (BAB-UF) reactors, where the incorporation of PVA significantly enhances structural integrity, biodegradation resistance, and functional lifespan, providing an efficient, sustainable solution for removing odorous compounds from rural water. Experimental results showed the enhanced chitosan/PVA porous biochar aerogels (CPPCA) displayed excellent biocapacity and structural stability.
View Article and Find Full Text PDFQuantum dots (QDs) are widely used, but their health impact on the visual system is little known. This study aims to elucidate the effects and mechanisms of typical metallic QDs on retinas using zebrafish. Comprehensive histology, imaging, and bulk RNA sequencing reveal that InP/ZnS QDs cause retinal degeneration.
View Article and Find Full Text PDFKitchen waste (KW) is an important reservoir of antibiotic resistance genes (ARGs). Black solider fly larvae (BSFL) are extensively employed in KW disposal, closely linking to their robust gut microbes. However, antibiotic resistome in BSFL gut during the KW disposal processes and the mechanism remain unclear.
View Article and Find Full Text PDFNon-alcoholic fatty liver disease (NAFLD), characterized by hepatic steatosis, is a common metabolic liver disease worldwide. Currently, satisfactory drugs for NAFLD treatment remain lacking. Obesity and diabetes are the leading causes of NAFLD, and compounds with anti-obesity and anti-diabetic activities are considered suitable candidates for treating NAFLD.
View Article and Find Full Text PDFObjective: This study was conducted to explore the differential diagnostic value of PET/computed tomography (PET/CT) combined with high-resolution computed tomography (HRCT) in predicting the invasiveness of ground-glass nodules (GGNs).
Materials And Methods: This retrospective analysis included 67 patients (mean age 62.5 ± 8.
Complement Ther Med
November 2024
Objectives: This meta-analysis aimed to explore the relationship between the dose of acupuncture sessions, acupuncture frequency, and acupuncture duration and its effects on migraine.
Methods: Eight databases were searched for randomized controlled trials (RCTs) evaluating the efficacy of penetrating manual acupuncture for migraine published in English and Chinese from inception to June 20, 2024. The robust-error meta-regression (REMR) approach and non-linear meta-regression with restricted cubic spline (RCS) were used to investigate the dose-response association between acupuncture sessions, acupuncture frequency, and acupuncture duration and the frequency of migraine attacks.
The reproduction and dispersal strategies of lichens play a major role in shaping their population structure and photobiont diversity. Sexual reproduction, which is common, leads to high lichen genetic diversity and low photobiont selectivity. However, the lichen genus adopts a special co-dispersal model in which algal cells from the photobiont and ascospores from the mycobiont are released together into the environment.
View Article and Find Full Text PDFBackground: The effectiveness and safety of acupuncture therapy to delay lung function decline in chronic obstructive pulmonary disease (COPD) remain unclear. This study aimed to determine whether acupuncture, as an adjunctive therapy to COPD-guided medication, could prevent lung function decline.
Methods: This randomised, two-centre study was conducted between February 2022 and July 2023.
Hepatocellular carcinoma (HCC) is a prevalent primary liver cancer often associated with chronic hepatitis B virus infection (CHB) and liver cirrhosis (LC), underscoring the critical need for biomarker discovery to improve patient outcomes. Emerging as a promising avenue for biomarker development, proteomic technology leveraging liquid biopsy from small extracellular vesicles (sEV) offers new insights. Here, we evaluated various methods for sEV isolation and identified polysaccharide chitosan (CS) as an optimal approach.
View Article and Find Full Text PDFThis study used the brilliant cresyl blue (BCB) staining method to group buffalo oocytes (BCB+ and BCB-) and perform in vitro maturation, in vitro fertilization and embryo culture. At the same time, molecular biology techniques were used to detect gap junction protein expression and oxidative stress-related indicators to explore the molecular mechanism of BCB staining to predict oocyte developmental potential. The techniques of buffalo oocytes to analyse their developmental potential and used immunofluorescence staining to detect the expression level of CX43 protein, DCFH-DA probe staining to detect ROS levels and qPCR to detect the expression levels of the antioxidant-related genes SOD2 and GPX1.
View Article and Find Full Text PDFBMC Public Health
July 2024