Engineering of Halide Methyltransferase BxHMT through Dynamic Cross-Correlation Network Analysis.

Halide methyltransferases (HMTs) provide an effective way to regenerate S-adenosyl methionine (SAM) from S-adenosyl homocysteine and reactive electrophiles, such as methyl iodide (MeI) and methyl toluene sulfonate (MeOTs). As compared with MeI, the cost-effective unnatural substrate MeOTs can be accessed directly from cheap and abundant alcohols, but shows only limited reactivity in SAM production. In this study, we developed a dynamic cross-correlation network analysis (DCCNA) strategy for quickly identifying hot spots influencing the catalytic efficiency of the enzyme, and applied it to the evolution of HMT from Paraburkholderia xenovorans.

[Functional peptidomics analysis of protein hydrolysates based on different enzyme treatments].

The aim of this study is to explore differences in the peptidomics of protein hydrolysates treated with different enzymes. Briefly, differences in the peptide fingerprints and active peptides of neutral protease/papain-hydrolyzed were analyzed using ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) combined with PEAKS Online 1.7 analysis software, Peptide Ranker, and the BIOPEP database.

[Treatment of lateral ankle joint ligament sprain by shaking and poking manipulation based on finite element method].

Objective: To conduct a preliminary study on joint injuries of anterior and calcaneal fibular ligaments of the lateral ankle joint, and to analyze mechanism of action of shaking and poking in treating ankle joint and biomechanical properties of ankle during the recovery of joint injuries.

Methods: CT scan was performed on a male volunteer with right ankle sprain. Mimics 10.

[Clinical observation of chiropractic manipulation in the treatment of degenerative scoliosis].

Objective: To observe clinical efficacy of chiropractic manipulation in the treatment of degenerative scoliosis (DS).

Methods: From June 2017 to September 2019, 120 patients with degenerative scoliosis were randomly divided into treatment group (60 cases) and control group(60 cases). The patients in treatment group were treated with chiropractic manipulation once every other day for 4 weeks.

[Influence of degenerative scoliosis on the treatment of lumbar spinal stenosis by MIS-TLIF].

Objective: To evaluate the effect of degenerative scoliosis on the difficulty and efficacy of minimally invasive transforaminal lumbar interbody fusion(MIS-TLIF) in the treatment of lumbar spinal stenosis.

Methods: From September 2016 to September 2019, 52 patients with lumbar spinal stenosis treated by MIS-TLIF were retrospectively analyzed, including 16 males and 36 females, aged from 42 to 71(63.44±5.

[Kinematic parameters and related influencing factors of sitting lumbar spine manipulation with motion capture].

Objective: To explore the influence of individual characteristics of patients on the kinematics parameters of sitting lumbar spine manipulation.

Methods: From January 2016 to December 2016, 30 patients with degenerative lumbar spondylolisthesis were treatede, including 18 males and 12 females, aged from 45 to 61(52.33±2.

Multifunctional modified polyvinyl alcohol: A powerful biomaterial for enhancing bioreactor performance in nitrate, Mn(II) and Cd(II) removal.

The co-existence of multiple pollutants in wastewater such as nitrate and heavy metal, is of high concern due to the potential environmental impact. In this study, a novel biomaterial PPy@FeO/PVA was synthesized as a multifunctional bacteria immobilized carrier, to enhance simultaneous denitrification, Cd(II) and Mn(II) removal efficiency in bioreactor environments. The morphology and main components of the PPy@FeO/PVA material were characterized by SEM and XRD.

Performance and microbial community of simultaneous removal of NO-N, Cd and Ca in MBBR.

A moving-bed biofilm reactor (MBBR) containing immobilized Acinetobacter sp.CN86 was operated to investigate the simultaneous denitrification, bio-mineralization and cadmium removal performance. Effects of hydraulic residence time (HRT) (4 h, 6 h and 8 h), pH (6.

Characterization of the Cd(II) and nitrate removal by bacterium Acinetobacter sp. SZ28 under different electron donor conditions.

In this study, zero-valent iron (ZVI), nanoscale zero-valent iron (nZVI), Fe(II), and Mn(II) were investigated for their effects on mixotrophic denitrification coupled with cadmium (Cd(II)) adsorption process by Acinetobacter sp. SZ28. The removal rates of nitrate were 0.

Analysis of the antimicrobial mechanism of porcine beta defensin 2 against E. coli by electron microscopy and differentially expressed genes.

Porcine beta defensin 2 (pBD2) is a cationic antimicrobial peptide with broad spectrum antibacterial activity, which makes it a potential alternative to antibiotics to prevent and cure diseases of pigs. However, development of pBD2 as an effective antibiotic agent requires molecular understanding of its functional mechanism against pathogens. In this study, we investigated the functional mechanism of pBD2 antibacterial activity.

[Analysis of X-ray signs of cervical spondylosis between vertebral artery type and radiculopathy].

Objective: To improve the X-ray diagnosis of cervical spondylosis of vertebral artery type (VCS).

Methods: A blinded design research. The X-ray signs both 60 patients with VCS and 60 patients with cervical spondylotic radiculopathy were collected from January 2011 to November 2012.

Antimicrobial characterization of site-directed mutagenesis of porcine beta defensin 2.

Porcine β defensin 2 (pBD2) is a small, cationic and amphiphilic antimicrobial peptide. It has broad antimicrobial activities against bacteria and plays an important role in host defense. In order to enhance its antimicrobial activity and better understand the effect of positively charged residues on its activity, we substituted eight amino acid residues with arginine or lysine respectively.

[Study on meteorological factors-based neural network model of malaria].

Objective: In order to provide reliable data for strategies development on prevention, a meteorological factors-based predicating model for malaria forecast was studied.

Methods: Data on malaria occurrence and climate changes from 1994 to 1999 in counties in Yunnan province was collected and analyzed with software packages of FoxPro 6.0 and Excel 5.