[Effects of propofol on myelin basic protein expression in oligodendrocytes of SD rats at different developmental stages].

Objective: To investigate the effect of propofol on myelin basic protein (MBP) expression in oligodendrocytes of SD rats at different developmental stages.

Methods: This study was conducted in 3?, 7?, 14? and 21?day?old SD rats (40 in each age group). In each group, the rats were randomized equally into control group and experimental group, and in the control group, the rats received an intraperitoneal injection of 25 mg/kg medium?long?chain fat emulsion followed by injections at a half dose every 20 min for 8 h; the rats in the experimental group were given injections of propofolmedium (at the initial dose of 25 mg/kg) in the same manner.

[Propofol suppresses invasion of human lung cancer A549 cells by down-regulating aquaporin-3 and matrix metalloproteinase-9].

Objective: To investigate the effect of propofol on cell invasion and expressions of aquaporin-3 (APQ-3) and matrix metalloproteinase-9 (MMP-9) in human lung adenocarcinoma cancer A549 cells.

Method: A549 cells were treated with propofol at the concentrations of 25, 50, and 100 µmol/L for 12 or 24 h. RT-PCR was used to detect the effect of propofol on AQP-3 mRNA level in A549 cells, and the effects of propofol treatments for 24 h on AQP-3 and MMP-9 protein expression and the invasive ability of A549 cells were assessed with Western blotting and Transwell assay, respectively.

[Propofol suppresses migration and invasion of breast cancer MDA-MB-231 cells by down-regulating H19].

Objective: To explore the effect of propofol on H19 expression, migration and invasion of human breast cancer MDA-MB-231 cells in vitro.

Methods: MDA-MB-231 cells were randomly divided into 5 groups for treatment with basal medium, DMSO, or propofol at concentrations of 25, 50, and 100 µmol/L. H19 expression of the treated cells was assessed with RT-PCR, and the changes of cell motility, migration and invasion were evaluated with wound-healing assay and Transwell assays.

Cardioprotective effect of novel sulphonamides-1,3,5-triazine conjugates against ischaemic-reperfusion injury via selective inhibition of MMP-9.

Diseases affecting cardiovascular system are ranked as a top most cause of morbidity and mortality. Herein, a novel class sulphonamides-1,3,5-triazine conjugates have been synthesized and tested for inhibitory activity against MMP-2 and MMP-9. The results of the study showed that these molecules efficiently inhibit MMP-9 than MMP-2, revealing compound 8e as the most potent inhibitor (IC  = 2.

Lutein protects against ischemia/reperfusion injury in rat skeletal muscle by modulating oxidative stress and inflammation.

Background: Lutein is an antioxidant compound with potential biological effects. The present study investigated the protective role of Lutein against I/R injury in skeletal muscle.

Methods: Animals were divided into three groups.

Propofol inhibits lipopolysaccharide-induced tumor necrosis factor-alpha expression and myocardial depression through decreasing the generation of superoxide anion in cardiomyocytes.

TNF-α has been shown to be a major factor responsible for myocardial depression in sepsis. The aim of this study was to investigate the effect of an anesthetic, propofol, on TNF-α expression in cardiomyocytes treated with LPS both in vivo and in vitro. In cultured cardiomyocytes, compared with control group, propofol significantly reduced protein expression of gp91phox and phosphorylation of extracellular regulated protein kinases 1/2 (ERK1/2) and p38 MAPK, which associates with reduced TNF-α production.

[Effect of propofol at uptake equilibrium on γ-aminobutyric acid in different cerebral regions in dogs].

Objective: To investigate the effect of propofol at doses for different anesthesia depths on γ-aminobutyric acid (GABA) in different cerebral regions at propofol uptake equilibrium in dogs.

Methods: Twelve 12-18-month-old healthy hybrid dogs weighing 10-12 kg were randomly divided into light anesthesia group (n=6) and deep anesthesia group (n=6) with a single bolus dose of propofol (5.5 and 7.

[Protective effects of penehyclidine hydrochloride against acute renal injury induced by hemorrhagic shock and lipopolysaccharides in rats].

Objective: To investigate the effect of penehyclidine hydrochloride (PHC) in a rat model of renal injury induced by hemorrhagic shock and lipopolysaccharides (LPS).

Methods: Forty-five healthy Wistar rats were randomized into sham operated group, model group, and 3 penehyclidine hydrochloride (PHC) dose (1, 2 and 3 mg/kg) groups (PHC1, PHC2, and PHC3 groups, respectively). The arterial blood samples were collected to determine the concentrations of serum tumor necrosis factor-α (TNF-α), interleukin-8 (IL-8), interleukin-1 (IL-1), urine creatinine (Cr) and blood urine nitrogen (BUN), and the renal tissues were collected to measure the expressions of ICAM-1 and nuclear factor-κB (NF-κB) and observe the pathological changes.

[Effect of noxious stimulation on regional distribution of propofol in canine spinal cord].

Objective: To observe the regional distribution of propofol in canine spinal cord under noxious stimulation.

Methods: Twelve healthy hybrid dogs (12-18 months old, weighing 10-12 kg) were randomly divided into control group (n=6) and stimulation group (n=6). All the dogs were anesthetized with a single bolus dose of propofol (7 mg/kg) in 15 seconds followed by propofol infusion at a constant rate of 70 mg/kg/h via the great saphenous vein of the right posterior limb.

[Prophylactic effect of acupuncture on nausea and vomiting after laparoscopic operation].

Objective: To explore the prophylactic effect of acupuncture Neiguan (PC 6) on nausea and vomiting after laparoscopic operation.

Methods: One hundred patients with laparoscopic gastrointestinal operation were randomly divided into an acupuncture group and a control group, 50 patients in each group. The operation was carried out with the combined infusion and inhalation anesthesia.

[Changes of approximate entropy in rats during focal cerebral infarction].

Objective: To investigate the changes of EEG approximate entropy (ApEn) in rats during focal cerebral infarction.

Methods: Twenty-four Sprague-Dawley (SD) rats were randomly divided into infarction group (n=12) with middle cerebral artery occlusion and sham-operated group (n=12). The EEG data (ApEn) was recorded in the bilateral areas (C3, C4) of the rats with focal cerebral infarction before the infarction and immediately and at 5, 15, 30, and 60 min after the infarction.

[Ulinastatin attenuates lung injury in rats with hemorrhagic shock].

Objective: To investigate the effects of ulinastatin on lung injury in hemorrhagic shock rats.

Methods: Twenty-four normal SD rats were randomly divided into 3 groups (n=8), namely the control group, hemorrhagic shock group (group H) and ulinastatin group (group U). In group H and group U, blood was drawn from the femoral artery over a period of 10 min until a mean arterial pressure of 40 mmHg was obtained.

[Cerebral uptake and regional cerebral distribution of propofol under concentration equilibrium condition in the internal carotid artery and internal jugular vein in dogs].

Objective: To investigate the cerebral uptake and regional distribution of propofol when plasma propofol concentration reaches equilibrium in the internal carotid artery and internal jugular vein in dogs.

Methods: Eight male hybrid dogs aged 12-18 months weighing 10-12 kg were anesthetized with propofol at a single bolus (7 mg/kg) in 15 s followed by propofol infusion at a constant rate of 70 mg.kg(-1).

[Cerebral distribution of propofol at cerebral propofol uptake equilibrium in dogs].

Objective: To investigate the cerebral distribution of propofol during continued infusion at a constant rate when the cerebral propofol uptake reaches equilibrium in dogs.

Methods: Six healthy 1-year-old male dogs were used in this study. The venous channel was established in the great saphenous vein of the right posterior limb.

[Protective effects of lidocaine against lung injury after hemorrhagic shock in rabbits].

Objective: To study the protective effect of lidocaine against lung injury after hemorrhagic shock in rabbits.

Methods: Eighteen healthy rabbits were randomly divided into 3 groups (n=6), namely lidocaine group (group L), hemorrhagic shock group (group H) and control group (group C). Hemorrhagic shock model was established in rabbits in groups L and H, and the venous blood samples were collected for measurement of plasma malondialdehyde (MDA) and superoxidedismutase (SOD) before phlebotomy (T0), 2 h after hemorrhagic shock (T1) and 2 h after resuscitation (T2).

[Patient-controlled intravenous analgesia with sufentanil and fentanyl after thoracotomy: a comparative study].

Objective: To evaluate the clinical efficacy of sufentanil and fentanyl at equivalent dose for patient-controlled intravenous analgesia (PCIA) after thoracotomy.

Methods: Sixty ASA I-II patients (20-60 years of age) undergoing radical operation for lung or esophageal cancer were randomly divided into sufentanil intravenous analgesia group (group S, with sufentanil 1 microg/ml) and fentanyl intravenous analgesia group (group F, fentanyl 10 microg/ml). PCIA was administered with background infusion of 2.

[Changes of oxygen cost of breathing in children and adult during anesthesia and surgery].

Objective: To investigate the changes of oxygen cost of breathing (OCB) in children and adult during general anesthesia and surgery.

Methods: This study included 12 elective surgical children and 12 adult patients receiving cerebral surgery. Mechanical ventilation was given during general anesthesia and surgery, and the indices of hemodynamics and respiratory function were measured at 5 min before induction, 1 min before the start of surgery, 30, 60, 120 and 180 min after the start of surgery and at the end of surgery, respectively.

[Ropivacaine or its combination with fentanyl for postoperative patient-controlled epidural analgesia].

Objective: To investigate the pain-relieving effects of ropivacaine or its combination with fentanyl in postoperative patient-controlled epidural analgesia.

Methods: Sixty patients (ASA class I to II) scheduled for elective upper abdominal surgery were randomly divided into 4 groups (15 in each group) to receive 2.0 mg/ml ropivacaine (group R), 1.

[Anesthetic management during resection of intratracheal tumor: report of 23 cases].

Objective: To summarize our experience in anaesthetic management during the resection of intratracheal tumor.

Methods: A retrospective analysis of the anaesthetic management was conducted in 23 cases of intratracheal tumor resection. The anaesthetic methods were determined according to the location of tumor, the degree of tracheal obstruction and the surgical procedures.

[Inhibiting effect of intrathecal fentanyl on intraoperative vomiting during cesarean delivery under epidural anesthesia].

Objective: To observe the inhibiting effect of intrathecal (IT) fentanyl on nausea and vomiting during cesarean delivery under epidural anesthesia.

Methods: Thirty healthy parturients (ASA grade I to II) were randomly assigned in equal numbers into control and fentanyl groups (n=15 each, patients in the latter group treated with IT fentanyl at the dose of 20 microgram/3ml). The incidence of nausea and vomiting during the elective cesarean delivery under epidural anesthesia between the 2 groups were compared and the neonates' Apgar scores assessed after the delivery.

Effects of patient-controlled upper and lower epidural analgesias on postoperative respiratory and circulatory function.

OBJECTIVE: To compare the effects of patient-controlled high thoracic epidural analgesia (PCHEA) and low thoracic epidural analgesia (PCLEA) on respiratory and circulatory functions after operation. METHODS: Thirty-six patients were divided into 2 groups with 18 in each, and one group (Group H) received PCHEA during thoracic operations and the other (Group L) received PCLEA during abdominal operations. Postoperative patient-controlled analgesia (PCA) was performed in all the patients with 0.

[Brain uptake of propofol during target-controlled infusion when effect compartment concentration is targeted].

Objective: To study the correlation between the effect compartment concentration (ECC) and the brain uptake of propofol during sedation by target-controlled infusion (TCI).

Method: Twelve ASA physical status I to II patients with neither cardiac nor intracranial diseases were scheduled for elective abdominal operation. Computer-assisted target-controlled infusion of propofol was performed for general anesthesia in all patients with the target ECC set at 4.

[Effects of intraperitoneal CO2 insufflation on hemodynamics and oxygen consumption during intravenous propofol anesthesia combined with epidural block].

Objective: To investigate the effects of intraperitoneal CO2 insufflation on the hemodynamics, oxygen consumption (VO2) and carbon dioxide production (VCO2) during intravenous anesthesia with propofol in combination with epidural block.

Methods: Intratracheal intubation was performed after rapid induction of anesthesia and mechanical ventilation was given. Maintenance of anesthesia was achieved using continuous intravenous propofol infusion (2 mg/kg/h) ?N2O inhalation and intermittent epidural administration.

Effect of patient-controlled epidural analgesia for pain relief after thymectomy in patients with myasthenia gravis.

Objective: To evaluate the effect of patient-controlled epidural analgesia (PCEA) with ropivacaine or bupivacaine in relieving pain after thymectomy in patients with myasthenia gravis.

Methods: Twenty adult ASA I-II patients with myasthenia gravis were randomized to receive either 0.125% ropivacaine (Group R, n=10) or 0.