Acute macular neuroretinopathy following COVID-19 infection: Three case reports.

Background: During the Coronavirus disease 2019 (COVID-19) pandemic, a notable increase in acute macular neuroretinopathy (AMN) cases was observed. This study aimed to investigate the potential association between AMN and COVID-19 by examining 3 cases in China.

Case Summary: The first case involved a 30-year-old man who presented with progressive vision loss following a COVID-19 infection.

One-Dimensional Selenidostannates Based on an Infrequent Tetrameric Cluster [SnSe] Exhibiting Electro-Catalytic Properties.

The discovery of low-cost and efficient electro-catalytic materials for hydrogen evolution reaction (HER) is very desirable in hydrogen energy technology. Here, a new type of one-dimensional (1-D) organic hybrid selenidostannate [Ni(en)][SnSe] (, en = ethylenediamine) with an [Ni(en)] complex was achieved by the solvothermal reaction of Sn, Se, and NiCl·6HO in a mixed solvent of en and triethanolamine at 160 °C for 10 days. The crystal structure of contains a unique 1-D [SnSe] chain built up from the sharing-edge connection of a hitherto-unknown tetrameric [SnSe] cluster, which is separated by discrete [Ni(en)] complexes.

Electro-catalytic Iodoplumbate Incorporating Two Types of Polymeric Anions.

The development of the most promising electro-catalysts for the high-performance hydrogen evolution reaction (HER) in neutral media is exceedingly desirable. Here, the convenient hydrothermal reaction of PbI, 3-pyrazinyl-1,2,4-triazole (3-pt), KI, and methanol in HI aqueous solution acquired an organic hybrid iodoplumbate [mtp][PbI][PbI]·0.5HO (denoted as , mtp = 3-(1,4-dimethyl-1-1,2,4-triazol-4-ium-3-yl)-1-methylpyrazin-1-ium), which not only provided an infrequent organic mtp cation that originated from the hydrothermal -methylation reaction of 3-pt in acidic KI solution but also offered the rare example of organic hybrid iodoplumbate incorporating both one-dimensional (1-D) [PbI] and two-dimensional (2-D) [PbI] polymeric anions with one configuration of the mtp cation.

Screening potential polyhydroxyalkanoate-producing bacteria from wastewater sludge.

We applied fluorescence staining of Nile red, polymerase chain reaction (PCR), and carbon substrate utilization and pressure tolerance analysis to execute three-stage screening for potential polyhydroxyalkanoate (PHA) producers in the sludge samples of 21 large-scale wastewater treatment plants of city and industrial parks in Taiwan area. Total 35,429 colonies were grown on 196 plates, the screened 30 strains were subjected to 16S rRNA analysis, and 18 identified genera belonged to Proteobacteria (67%), Firmicutes (17%), and Actinomycetota (16%). The PHA accumulation results revealed that nine genera (50% of 18 screened) produced PHAs by limiting the nitrogen source and excess single carbon sources of glucose in an aerobic status.

Infrequent Cubane-Like Chromium Sulfide Cluster with σ-Donor Ligands with Efficient Electrocatalytic Property Toward Hydrogen Evolution Reaction.

The exploitation of efficient and economical electrocatalysts for hydrogen evolution reaction (HER) is of exceeding interest in renewable clean-energy technologies. Herein, the facile solvothermal reaction of S and chromic acetate in ethylenediamine (en) achieved a novel organic hybrid chromium sulfide [Cr(μ-S)(en)(SH)]·0.25HO (), which offers a new type of antiferromagnetic cubane-like chromium sulfide cluster with σ-donor en ligands.

One Luminescent Cadmium Iodide with Free Bifunctional Azole Sites as a Triple Sensor for Cu, Fe, and CrO Ions.

The exploration of an excellent triple sensor for monitoring Cu, Fe, and CrO ions is of exceeding significance because of their serious effects on the human body. Herein, optically active 1H-3,5-bis(pyrazinyl)-1,2,4-triazole (Hbpt) with triazolyl and pyrazinyl groups was applied for the construction of a new type of organic hybrid cadmium iodide [CdI(bpt)(HO)]·2HO () incorporating a hitherto-unknown [CdI(HO)] trimeric-cationic unit, which shows an orange light emission at 589 nm with a large Stokes shift of 246 nm. In virtue of the existence of free bifunctional azole sites as the receptors, exhibits a highly selective and sensitive sensing property toward Cu, Fe, and CrO ions in aqueous solution with lower detection limits of 0.

The optimal combination of Nile red identification, colony polymerase chain reaction, and gas chromatography detection methods in screening for polyhydroxyalkanoicate-producing bacteria.

The study devised a detection process combining Nile red-containing media, polymerase chain reaction (PCR), and gas chromatography (GC) to evaluate the possibility of microbes becoming polyhydroxyalkanoate (PHA) producers. The Nile red and PCR detection steps of designating PHA producers had true positive rates of 39.4% and 40%, respectively, and the use of GC analysis as the final step yielded accurate results for the production types and yields of PHAs.

Prognostic significance of soluble CD25 in patients with sepsis: a prospective observational study.

Objectives: The diagnosis of sepsis is challenging, the need for sensitive and specific diagnostic and prognostic biomarkers has not been met. Soluble CD25 (sCD25) is a readily available biomarker reported to represent the severity of the disease. This study aimed to assess the association between sCD25 and mortality in patients with sepsis.

Corrigendum: Associations of Serum S100A12 With Severity and Prognosis in Patients With Community-Acquired Pneumonia: A Prospective Cohort Study.

[This corrects the article DOI: 10.3389/fimmu.2021.

Effect of early restrictive fluid resuscitation on inflammatory and immune factors in patients with severe pelvic fracture.

Purpose: To study the effect of early restrictive fluid resuscitation (EFR) on inflammatory and immune factors in patients with severe pelvic fracture (SPF).

Methods: A total of 174 SPF patients in the Department of Orthopaedics, the First Affiliated Hospital of Chengdu Medical College from July 2015 to June 2018 were involved in this study and divided into EFR group (n = 87) and control group (n = 87) using the random number table method. Conventional fluid resuscitation (CFR) was performed in control group, and EFR was performed in EFR group.

Gastrodin inhibits high glucose‑induced human retinal endothelial cell apoptosis by regulating the SIRT1/TLR4/NF‑κBp65 signaling pathway.

Diabetic retinopathy (DR), one of the most common complications of late‑phase diabetes, is associated with the ectopic apoptosis of microvascular cells. Gastrodin, a phenolic glucoside derived from Gastrodia elata Blume, has been reported to have antioxidant and anti‑inflammation activities. The aim of the present study was to investigate the effects of gastrodin on high glucose (HG)‑induced human retinal endothelial cell (HREC) injury and its underlying mechanism.

Immobilization of uranium into magnetite from aqueous solution by electrodepositing approach.

Immobilization of uranium into magnetite (FeO), which was generated from metallic iron by electrochemical method, was proposed to rapidly remove uranium from aqueous solution. The effects of electrochemical parameters such as electrode materials, voltage, electrode gap, reaction time and pH value on the crystallization of FeO and uranium removal efficiencies were investigated. More than 90% uranium in the solution was precipitated with FeO under laboratory conditions when uranium concentration range from 0.

Effects of Ulinastatin on In Vitro Storage Lesions of Human Red Blood Cells.

Background: The study aimed to investigate the influence of in vitro storage on erythrocyte complement receptor one (E-CR1), cell shrinkage and eryptosis of human red blood cells (RBCs), and to assess the possible effects of ulinastatin (UTI) on them.

Methods: After collection, RBCs were treated with saline (control group) and different concentrations of UTI (5,000 U/mL, 10,000 U/mL, and 50,000 U/mL in Group C1, Group C2, and Group C3, respectively). E-CR1, cell size, and phosphatidylserine (PS) exposure and intracellular Ca2+ concentration were analyzed by flow cytometer every 7 days up to Day 35.

Differentially expressed miRNAs in sepsis-induced acute kidney injury target oxidative stress and mitochondrial dysfunction pathways.

Objective: To identify specific miRNAs involved in sepsis-induced AKI and to explore their targeting pathways.

Methods: The expression profiles of miRNAs in serum from patients with sepsis-induced AKI (n = 6), sepsis-non AKI (n = 6), and healthy volunteers (n = 3) were investigated by microarray assay and validated by quantitative PCR (qPCR). The targets of the differentially expressed miRNAs were predicted by Target Scan, mirbase and Miranda.

Inhibition of TLR4 protects rat islets against lipopolysaccharide-induced dysfunction.

Oxidative stress leads to dysfunction in pancreatic cells, causing a reduction in insulin secretion following exposure to glucose. Toll-like receptor 4 (TLR4) may be activated by exposure to lipopolysaccharide (LPS) stress. TLR4 may mediate the initiation of inflammatory and immune defense responses; however, the importance of the LPS/TLR4 interaction in apoptosis induced by oxidative stress in pancreatic β cells remains to be elucidated.

[Research on output and quality of Panax notoginseng and annual change characteristics of N, P and K nutrients of planting soil under stereo-cultivation].

The output and agronomic characters of 3-year-old Panax notoginseng cultured under stereo structure (upper, middle and down layers) were investigated, and the annual change of N, P and K of its planting soil were also studied. Results showed that, compared with field cultured Panax notoginseng, growth vigour and output of stereo-cultivation were significantly lower. But the total production of the 3 layers was 1.

Functional analysis of differentially expressed proteins in Chinese bayberry (Myrica rubra Sieb. et Zucc.) fruits during ripening.

This study developed a proteome reference map of Myrica rubra fruits at the green, pink and red stages during ripening using two-dimensional gel electrophoresis (2-DE). Forty-six differentially expressed proteins were detected in the gel, of which 43 were successfully identified by matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry and protein database searching. We found that malic enzyme related to the decrease of organic acid acidity was up-regulated.

Expression of cytokines in mouse hepatitis B virus X gene-transfected model.

The expression profile in the mouse hepatitis B virus X (HBx)-transfected model was investigated in order to lay a foundation for further study on the implication of cytokines expression in hepatitis B virus (HBV) infection. Hydrodynamic injection method via the tail vein was used to establish the animal HBx-transfected model. By using microassay, the differential expression of gene in each group was analyzed, which was further confirmed by using real-time PCR and semi-quantitative PCR.

Assessment of a five-color flow cytometric assay for verifying automated white blood cell differentials.

Background: White blood cell (WBC) counts and differentials performed using an automated cell counter typically require manual microscopic review. However, this last step is time consuming and requires experienced personnel. We evaluated the clinical efficiency of using flow cytometry (FCM) employing a six-antibody/five-color reagent for verifying automated WBC differentials.

Chemotherapy and radiotherapy downregulate the activity and expression of DNA methyltransferase and enhance Bcl-2/E1B-19-kDa interacting protein-3-induced apoptosis in human colorectal cancer cells.

Bcl-2/E1B 19-kDa interacting protein 3 (BNIP3) is a proapoptotic protein whose expression level is often low in colorectal cancer (CRC) cells due to the BNIP3 gene promoter DNA methylation by DNA methyltransferase (DNMT). It is known that chemotherapy and radiotherapy suppress CRC through inducing tumor apoptosis. However, the molecular mechanisms underlying chemotherapy and radiotherapy-induced apoptosis of CRC cells are not well defined.

Can circulating interleukin-18 differentiate between sarcoidosis and idiopathic pulmonary fibrosis?

Objective: The aim of the present study was to investigate serum and bronchoalveolar lavage fluid (BALF) interleukin-18 (IL-18) levels in sarcoidosis and idiopathic pulmonary fibrosis (IPF) and to assess the clinical usefulness of IL-18 in these diseases.

Methods: Serum and BALF levels of IL-18 were measured in 15 patients with sarcoidosis, 10 patients with IPF and 24 control subjects (8 with lung tumor, 6 with pulmonary tuberculosis and 10 healthy controls). Lymphocyte fractions (T, B and natural killer (NK) cells) in blood and BALF were analysed by flow cytometer.

Effects of the proapoptotic regulator Bcl2/adenovirus EIB 19 kDa-interacting protein 3 on radiosensitivity of cervical cancer.

Purpose: Bcl2/adenovirus EIB 19 kDa-interacting protein 3 (BNIP3) is a proapoptotic member of the Bcl-2 family. To address its potential as a therapeutic target for radiosensitization, this study investigated the effect of Bnip3 expression on radiosensitivity of cervical cancer in vitro and in vivo.

Materials And Methods: In vitro: A plasmid expressing the BNIP3 gene was transfected into human cervical cancer HeLa cells using Lipofectamine(2000), and western blot and immunohistochemistry analysis were performed to evaluate the expression of BNIP3 in transfected cells.

[Effect and mechanism of DNMT inhibitor on the reversal of multidrug resistance in human colon cancer cell line sw620/L-OHP].

Objective: To explore whether demethylation inhibitor 5-aza-2'-deoxycytidine (5-aza-dC) could reverse multidrug resistance (MDR) of human colon cancer and its impacts on the expressions of Bcl-2/adenovirus E1B 19 KDa protein interacting protein 3 (BNIP3), P-glycoprotein (P-GP) and multidrug resistance-associated protein (MRP), as well as the activity of DNA methyltransferase (DNMT).

Methods: Oxaliplatin (L-OHP) resistant human colon carcinoma cells (sw620/L-OHP) and its parental cells (sw620) were used to determine the effect of 5-aza-dC. CCK-8 assay was adopted to evaluate the cytotoxicity of 5-aza-dC.