Recurrent SARS-CoV-2 RNA positivity and prolonged viral shedding in a patient with COVID-19: a case report.

Background: The ongoing coronavirus disease 2019 (COVID-19) global pandemic caused by the SARS-CoV-2 virus remains a major threat to public health. At present, it is recommended that patients with known or suspected COVID-19 undergo quarantine or medical observation for 14 days. However, recurrent SARS-CoV-2 RNA positivity and prolonged viral shedding have been documented in convalescent COVID-19 patients, complicating efforts to control viral spread and ensure patient recovery.

[Research of influence factors on spectral recognition for cotton leaf infected by Verticillium wilt].

Through carrying out spectral test experiment, the influence factors of spectrum test were analyzed, the influence degree of various factors in spectral recognition was explicated and the method of spectra test was optimized for cotton leaf infected by verticillium wilt. The results indicated that under different severity levels, the shape and value of reflectance of disease symptoms part were Significantly higher than healthy part on cotton leaf, compared with the black board as baseboard, the spectral values of disease leaves were slightly higher in visible light wavebands and significantly higher in others wavebands than healthy leaves on white baseboard. Different position of leaf on cotton plant has different effect degree to the recognition of disease, the effect of stem leaf was more obvious than that of else leaf, the identical leaf position was less influenced by disease than band.

Estimation of wheat agronomic parameters using new spectral indices.

Crop agronomic parameters (leaf area index (LAI), nitrogen (N) uptake, total chlorophyll (Chl) content ) are very important for the prediction of crop growth. The objective of this experiment was to investigate whether the wheat LAI, N uptake, and total Chl content could be accurately predicted using spectral indices collected at different stages of wheat growth. Firstly, the product of the optimized soil-adjusted vegetation index and wheat biomass dry weight (OSAVI×BDW) were used to estimate LAI, N uptake, and total Chl content; secondly, BDW was replaced by spectral indices to establish new spectral indices (OSAVI×OSAVI, OSAVI×SIPI, OSAVI×CIred edge, OSAVI×CIgreen mode and OSAVI×EVI2); finally, we used the new spectral indices for estimating LAI, N uptake, and total Chl content.

[Estimated nitrogen nutrition index based on the hyperspectral for wheat of drip irrigation under mulch].

The accurate wheat management needs a reasonable nitrogen application, and it is one of the key measures for real-time and quantitatively monitoring of nitrogen status to gain the higher yield of wheat. In the present study, two field experiments were conducted with different nitrogen stress and wheat cultivars, the relationship was analyzed between spectral parameters and the partial factor productivity from applied N (PFPn), and the estimating model was established for PFP, in the growth stages of wheat. The result indicated that there was a highly significant correlation between the PFP, and GreenNDVI at jointing, the correlation coefficient (r) was 0.

NEFL mRNA expression level is a prognostic factor for early-stage breast cancer patients.

Neurofilament, light polypeptide (NEFL) was demonstrated to be ectopically expressed in breast cancer tissues and decreased in lymph node metastases compared to the paired primary breast cancers in our previous study. Moreover, in several studies, NEFL was regarded as a tumor suppressor gene, and its loss of heterozygosity (LOH) was related to carcinogenesis and metastasis in several types of cancer. To explore the role of NEFL in the progression of breast cancer and to evaluate its clinical significance, we detected the NEFL mRNA level in normal breast tissues, primary breast cancer samples and lymph node metastases, and then analyzed the association between the NEFL expression level and several clinicopathological parameters and disease-free survival (DFS).

[Application of latissimus dorsi muscle flap for breast conservation in breast cancer].

Objective: Breast conserving surgery (BCS) is one of standard treatment approaches in early breast cancer. Although most defect after BCS can be repaired, the cosmetic outcomes are unsatisfactory in the patients with poor tumor/breast ratio. Oncoplastic surgery (OPS) has emerged as a new approach for providing adequate tumor resection without compromise of aesthetic outcomes in BCS.

[Monitoring canopy nitrogen status in winter wheat of growth anaphase with hyperspectral remote sensing].

Biomass, leaf area index (LAI) and nitrogen status are important parameters for indicating crop growth potential and photosynthetic productivity in wheat. Nondestructive, quick assessment of leaf dry weight, LAI and nitrogen content is necessary for nitrogen nutrition diagnosis and cultural regulation in wheat production. In order to establish the monitoring model of nitrogen richness in winter wheat of growth anaphase, studying the relationship between the nitrogen richness (NR) containing nitrogen density, LAI and leaf dry weight and the difference of hyperspectral reflectance rates (deltaR), we conducted a comparable experiment with five winter wheat varieties under nitrogen application level of 0, 100, 200 and 400 kg x N x ha(-1).

[Study on hyperspectral estimation of pigment contents in leaves of cotton under disease stress].

The spectrum reflectance and pigment contents of cotton leaves infected with Verticillium wilt were measured in cotton disease nursery and field in different growth phases, and severity level of Verticillium wilt was investigated. The correlation between pigment contents of cotton leaves with Verticillium wilt and spectra reflectance, the first derivative of reflectance and spectral characteristic parameters were analyzed respectively. The estimation models about leaves pigment contents of disease cotton were established and tested.

[Research on the postoperative complications and its risk factors of breast reconstruction with abdominal flaps].

Objective: To study the postoperative complications and its risk factors in patients underwent breast reconstruction with abdominal flaps.

Methods: The clinical data of 115 cases underwent breast reconstructions with abdominal flaps from May 2001 to October 2008 was reviewed. The postoperative complications included total flap necrosis, partial flap necrosis, fat necrosis, hernia, bulge, fat liquefaction and infection.

[Application of latissimus dorsi-myocutaneous flap in breast reconstruction].

Objective: To investigate the design, procedures and effect of latissimus dorsi-myocutaneous flap (LDMF) for breast reconstruction.

Methods: From May, 2005 to April, 2006, 18 consecutive patients underwent breast reconstruction with LDMF. Combined breast implants were also inserted in 8 of the 18 cases.

[Clinical significance and relationship of expression of matrix metalloproteinase-2 mRNA with breast cancer metastasis].

Objective: To discuss the clinical significance of matrix metalloproteinase-2 (MMP-2) mRNA and the relationship of MMP-2 mRNA expression with breast cancer metastasis.

Method: Detect MMP-2 mRNA expression of 30 breast cancer, metastatic lymphnode and 116 clinical breast cancer samples by flurence-quantitative RT-PCR and analysis the relativity of MMP-2 mRNA expression with clinical, pathology factors.

Results: MMP-2 mRNA expression in 30 metastatic lymphnode is lower than in primary breast cancer (t = -3.

[Screening of differential expression genes between primary breast cancer and its lymph node metastasis using single primer amplification of cDNA for microarray].

Objective: To screen genes related to breast cancer metastasis by comparing difference of expression profile between primary breast cancer and its lymph node metastasis.

Methods: The cell total RNA was extracted from 10 breast cancer specimens, including the primary cancer and metastatic cancer tissues of axillary lymph nodes resected during operation. Single primer amplification (SPA) was used to prepare fluorescence-labeled targets and then Oligo microarray concluding 21 000 human functional genes was used to screen out 1.